Please use this identifier to cite or link to this item: http://repositorio.unicamp.br/jspui/handle/REPOSIP/326103
Type: Artigo
Title: A Survey Of The Peptide Profile In Prato Cheese As Measured By Maldi-ms And Capillary Electrophoresis
Author: Baptista
Debora Parra; da Silva Araujo
Francisca Diana; Eberlin
Marcos Nogueira; Gigante
Mirna Lucia
Abstract: In this study, we describe the characterization of the peptide profile in commercial Prato cheese by matrix-assisted laser desorption ionization mass spectrometry (MALDI-MS) and capillary electrophoresis (CE). Ten commercial Prato cheese brands were characterized via their physicochemical composition and subjected to fractionation according to solubility at pH 4.6. The pH 4.6 insoluble fraction was evaluated by CE, whereas MALDI-MS was applied to the fraction soluble at pH 4.6 and in 70% ethanol. CE revealed a characteristic pattern of hydrolysis, with formation of para--casein, hydrolysis of (s1)-casein at the Phe(23) - Phe(24) bond, and hydrolysis of -casein. For the MALDI-MS data, a complex peptide profile was observed, with the identification of 44 peptides previously reported (24 peptides from (s1)-casein, 14 from -casein, 3 from -casein, and 3 from (s2)-casein). It was also observed that cheeses with salt-in-moisture content greater than 5% showed an accumulation of a bitter-tasting peptide (m/z 1536, (s1)-CN f1-13), suggesting a relationship between the higher salt concentration and the abundance of this peptide. In conclusion, the results showed that even commercial cheeses produced with different raw material and processing conditions showed very similar peptide profiles when assessed at the molecular level, and only 9 peptides were responsible for discrimination of cheeses.
Subject: Capillary Electrophoresis
Chemometrics
Maldi-ms
Prato Cheese
Proteolysis
Editor: Wiley-Blackwell
Hoboken
Citation: Journal Of Food Science. Wiley-blackwell, v. 82, p. 386 - 393, 2017.
Rights: fechado
Identifier DOI: 10.1111/1750-3841.13618
Address: http://onlinelibrary-wiley-com.ez88.periodicos.capes.gov.br/doi/10.1111/1750-3841.13618/full
Date Issue: 2017
Appears in Collections:Unicamp - Artigos e Outros Documentos

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