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Type: Artigo
Title: Acute Exercise Restores Insulin Clearance In Diet-induced Obese Mice
Author: Kurauti
Mirian A.; Costa-Junior
Jose M.; Ferreira
Sandra M.; dos Santos
Gustavo J.; Protzek
Andre O. P.; Nardelli
Tarlliza R.; de Rezende
Luiz F.; Boschero
Antonio C.
Abstract: The aim of this study was to investigate the insulin clearance in diet-induced obese (DIO) mice submitted to acute endurance exercise (3 h of treadmill exercise at 60-70% VO(2)max). Glucose-stimulated insulin secretion in isolated islets; ipGTT; ipITT; ipPTT; in vivo insulin clearance; protein expression in liver, skeletal muscle, and adipose tissue (insulin degrading enzyme (IDE), insulin receptor subunit beta (IR beta), phospho-Akt (p-Akt) and phospho-AMPK (p-AMPK)), and the activity of IDE in the liver and skeletal muscle were accessed. In DIO mice, acute exercise reduced fasting glycemia and insulinemia, improved glucose and insulin tolerance, reduced hepatic glucose production, and increased p-Akt protein levels in liver and skeletal muscle and p-AMPK protein levels in skeletal muscle. In addition, insulin secretion was reduced, whereas insulin clearance and the expression of IDE and IR beta were increased in liver and skeletal muscle. Finally, IDE activity was increased only in skeletal muscle. In conclusion, we propose that the increased insulin clearance and IDE expression and activity, primarily, in skeletal muscle, constitute an additional mechanism, whereby physical exercise reduces insulinemia in DIO mice.
Subject: High-fat Diet
Insulin Degrading Enzyme
Editor: Bioscientifica Ltd
Rights: fechado
Identifier DOI: 10.1530/JOE-15-0483
Date Issue: 2016
Appears in Collections:Unicamp - Artigos e Outros Documentos

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