Please use this identifier to cite or link to this item: http://repositorio.unicamp.br/jspui/handle/REPOSIP/325273
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dc.contributor.CRUESPUNIVERSIDADE DE ESTADUAL DE CAMPINASpt_BR
dc.typeArticlept_BR
dc.titleA Multiparametric Assay To Compare The Cytotoxicity Of Different Storage Media For Avulsed Teethen
dc.contributor.authorEmmanuel João Nogueira Leal Silvapt_BR
dc.contributor.authorAlexandre Augusto Zaiapt_BR
unicamp.authorEmmanuel João Nogueira Leal Silva - UNICAMP, Alexandre Augusto Zaia - UNICAMPpt_BR
dc.subjectTooth Avulsionen
dc.subjectFibroblastsen
dc.subjectCell Viabilityen
dc.description.abstractAim: To evaluate the cytotoxicity of several storage media (coconut water, whole milk, isotonic solution, Hank´s Balanced Salt Solution and tap water) using a multiparametric cytotoxicity analysis employing 3T3 cells. Methods: Plates containing confluent 3T3 fibroblasts were exposed to the various media for 24h, at 37°C with 5% CO2 , and cell viability was evaluated by a multiparametric assay assessing sequentially, on the same cells, mitochondrial activity (XTT), membrane integrity (neutral red test), and total cell density (crystal violet dye exclusion test). Results from each test were compared by one-way analysis of variance (ANOVA). Results: Statistical analysis showed that whole milk and HBSS were the most effective media in maintaining cell viability at all tested times (p<0.05). Isotonic and tap water showed the highest cytotoxicity effects. Conclusions: This study shows that whole milk and HBSS are more efficient in maintaining the viability of 3T3 fibroblasts as demonstrated by three different cell viability tests.en
dc.relation.ispartofBrazilian Journal of Oral Sciencespt_BR
dc.relation.ispartofabbreviationbjospt_BR
dc.date.issued2013pt_BR
dc.identifier.citationBrazilian Journal of Oral Sciences, v. 12, n. 2, p. 90-94pt_BR
dc.description.volume12pt_BR
dc.description.issuenumber2pt_BR
dc.description.lastpage90-94pt_BR
dc.rightsabertopt_BR
dc.sourcePPECpt_BR
dc.identifier.eissn1677-3225pt_BR
dc.identifier.doi10.20396/bjos.v12i2.8641064pt_BR
dc.identifier.urlhttps://periodicos.sbu.unicamp.br/ojs/index.php/bjos/article/view/8641064pt_BR
dc.date.available2017-09-12T14:31:52Z-
dc.date.accessioned2017-09-12T14:31:52Z-
dc.description.provenanceMade available in DSpace on 2017-09-12T14:31:52Z (GMT). No. of bitstreams: 0 Previous issue date: 2013en
dc.identifier.urihttp://repositorio.unicamp.br/jspui/handle/REPOSIP/325273-
dc.identifier.idPPEC8641064pt_BR
Appears in Collections:Unicamp - Artigos e Outros Documentos

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