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Type: Artigo
Title: Como Estudar Interações Entre Nanopartículas E Sistemas Biológicos
Title Alternative: Studying The Interactions Between Nanoparticles And Biological Systems
Author: Radaic
Allan; Pugliese
Gustavo Oliveira; Campese
Guilherme Capiraço; Pessine
Francisco Benedito Teixeira; Jesus
Marcelo Bispo de
Abstract: Although in recent years there has been an increasing amount of literature on nanotechnology and their clinical applications, it is still scarce a deep understanding of the interactions at the molecular levels between nanoparticles and cells. Studies demonstrating the underlying mechanisms of nanoparticles endocytosis, intracellular trafficking, and cellular processing are imperative to understand better how cells interact with those materials and their possible undesired effects, e.g. nanotoxicity. The rising awareness concerning nanoparticles applications and its interactions with the cellular environment is part of the new research field called Nanotoxicology. The cumulative knowledge in nanotoxicology will allow us to foresee toxic effects, establish regulations and limits for nanoparticles applications. In this work, we discuss the theoretical concepts about studying endocytosis and intracellular trafficking of nanoparticles. The nanoparticles-cell interactions are a multi-step process, which can be divided into nanoparticles' internalization, intracellular processing and triggering effects of nanomaterials on eukaryotic cells. Finally, we discuss the main techniques used to study this process: flow cytometry, use of endocytosis inhibitors and confocal microscopy.
Subject: Endocytosis
Intracellular Trafficking
Nanoparticles, Nanotoxicology
Nano-cell Interactions
Editor: Sociedade Brasileira de Química
Rights: aberto
Date Issue: 2016
Appears in Collections:Unicamp - Artigos e Outros Documentos

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