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Type: Artigo
Title: Cromatografia Por Interações Hidrofilicas (hilic): Estado Da Arte E Aplicações
Title Alternative: Hydrophilic Interaction Chromatography (hilic): State Of The Art And Applications
Author: Silva
Carla Grazieli Azevedo da; Bottoli
Carla Beatriz Grespan; Collins
Carol H.
Abstract: Hydrophilic interaction liquid chromatography (HILIC) has been gaining increased attention for its effective separation of highly polar compounds, which include carbohydrates, amino acids, pharmaceutical compounds, proteins, glycoproteins, nucleosides, etc. Polar compounds are usually poorly retained on reverse-phase liquid chromatography (RP-HPLC) columns or have poor solubility in the apolar mobile phase of normal-phase high performance liquid chromatography (NP-HPLC). Since HILIC uses organic solvents such as ACN or MeOH ( > 70%), also used in RP-HPLC and polar stationary phases similar to NP-HPLC (bare silica, diol, amino, amide, saccharide, zwitterionic stationary phases, etc.), it represents a hybrid of the two separation modes. The high organic content in the MP leads to good compatibility with mass spectrometry (MS), increasing the detectivity. This review describes the fundamentals of HILIC and highlights some interesting applications.
Subject: Hydrophilic Interaction Chromatography
Separation Of Polar Compounds
Hilic Separations
High Performance Liquid Chromatography
Polar Stationary Phases
Editor: Sociedade Brasileira de Química
Citation: Química Nova. Sociedade Brasileira De Química, v. 39, n. 2, p. 210 - 220
Rights: aberto
Date Issue: 2016
Appears in Collections:Unicamp - Artigos e Outros Documentos

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