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dc.contributor.CRUESPUNIVERSIDADE DE ESTADUAL DE CAMPINASpt_BR
dc.typeArtigopt_BR
dc.titleProspecting For The Incidence Of Genes Involved In Ochratoxin And Fumonisin Biosynthesis In Brazilian Strains Of Aspergillus Niger And Aspergillus Welwitschiaeen
dc.contributor.authorMassi F.P.pt_BR
dc.contributor.authorSartori D.pt_BR
dc.contributor.authorde Souza Ferranti L.pt_BR
dc.contributor.authorIamanaka B.T.pt_BR
dc.contributor.authorTaniwaki M.H.pt_BR
dc.contributor.authorVieira M.L.C.pt_BR
dc.contributor.authorFungaro M.H.P.pt_BR
unicamp.author.externalCentro de Ciências Biológicas, Universidade Estadual de Londrina, P. O. Box 6001, Londrina Zip Code, Brazilpt_BR
unicamp.author.externalInstituto de Tecnologia de Alimentos, P.O. Box 139, Campinas Zip Code, Brazilpt_BR
unicamp.author.externalDepartamento de Genética, Escola Superior de Agricultura Luiz de Queiroz USP, P.O. Box 83, Piracicaba Zip Code, Brazilpt_BR
dc.subjectA. Nigeren
dc.subjectA. Welwitschiaeen
dc.subjectFumonisin B2en
dc.subjectGene Deletionsen
dc.subjectMultiplex Pcren
dc.subjectOchratoxin Aen
dc.description.abstractAspergillus niger "aggregate" is an informal taxonomic rank that represents a group of species from the section Nigri. Among A. niger "aggregate" species Aspergillus niger sensu stricto and its cryptic species Aspergillus welwitschiae (=Aspergillus awamori sensu Perrone) are proven as ochratoxin A and fumonisin B2 producing species. A. niger has been frequently found in tropical and subtropical foods. A. welwitschiae is a new species, which was recently dismembered from the A. niger taxon. These species are morphologically very similar and molecular data are indispensable for their identification. A total of 175 Brazilian isolates previously identified as A. niger collected from dried fruits, Brazil nuts, coffee beans, grapes, cocoa and onions were investigated in this study. Based on partial calmodulin gene sequences about one-half of our isolates were identified as A. welwitschiae. This new species was the predominant species in onions analyzed in Brazil. A. niger and A. welwitschiae differ in their ability to produce ochratoxin A and fumonisin B2. Among A. niger isolates, approximately 32% were OTA producers, but in contrast only 1% of the A. welwitschiae isolates revealed the ability to produce ochratoxin A. Regarding fumonisin B2 production, there was a higher frequency of FB2 producing isolates in A. niger (74%) compared to A. welwitschiae (34%). Because not all A. niger and A. welwitschiae strains produce ochratoxin A and fumonisin B2, in this study a multiplex PCR was developed for detecting the presence of essential genes involved in ochratoxin (polyketide synthase and radHflavin-dependent halogenase) and fumonisin (α-oxoamine synthase) biosynthesis in the genome of A. niger and A. welwitschiae isolates. The frequency of strains harboring the mycotoxin genes was markedly different between A. niger and A. welwitschiae. All OTA producing isolates of A. niger and A. welwitschiae showed in their genome the pks and radH genes, and 95.2% of the nonproducing isolates did not contain these genes. The α-oxoamine synthase gene was detected in 100% and 36% of the A. niger and A. welwitschiae isolates, respectively. The loss of ochratoxin A production in A. niger and A. welwitschiae is highly associated with gene deletions within the ochratoxin biosynthetic gene cluster. The loss of fumonisin production in A. welwitschiae is associated with gene deletions within the fumonisin biosynthetic gene cluster, but this is not the case with A. niger. © 2016.en
dc.relation.ispartofInternational Journal of Food Microbiologypt_BR
dc.publisherElsevier B.V.pt_BR
dc.date.issued2016pt_BR
dc.identifier.citationInternational Journal Of Food Microbiology. Elsevier B.v., v. 221, p. 19 - 28, 2016.pt_BR
dc.language.isoEnglishpt_BR
dc.description.volume221pt_BR
dc.description.initialpage19pt_BR
dc.description.lastpage28pt_BR
dc.rightsfechadopt_BR
dc.sourceScopuspt_BR
dc.identifier.issn0168-1605pt_BR
dc.identifier.doi10.1016/j.ijfoodmicro.2016.01.010pt_BR
dc.identifier.urlhttps://www.scopus.com/inward/record.uri?eid=2-s2.0-84954548125&doi=10.1016%2fj.ijfoodmicro.2016.01.010&partnerID=40&md5=bcf0f1a27d8cec69493a76fe0e20eedfpt_BR
dc.date.available2017-08-17T19:17:46Z-
dc.date.accessioned2017-08-17T19:17:46Z-
dc.description.provenanceMade available in DSpace on 2017-08-17T19:17:46Z (GMT). No. of bitstreams: 1 2-s2.0-84954548125.pdf: 731429 bytes, checksum: 80bcd8f281374ae6e354b8512820b9b4 (MD5) Previous issue date: 2016en
dc.identifier.urihttp://repositorio.unicamp.br/jspui/handle/REPOSIP/324160-
dc.identifier.idScopus2-s2.0-84954548125pt_BR
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