Please use this identifier to cite or link to this item: http://repositorio.unicamp.br/jspui/handle/REPOSIP/323320
Type: Artigo
Title: A Highly Polar Phytocomplex Involving Rutin Is Responsible For The Neuromuscular Facilitation Caused By Casearia Sylvestris (guaçatonga)
Author: Yoshida E.H.
Tribuiani N.
Foramiglio A.L.
Foramiglio C.A.
Da Silva Tavares R.V.
Bonomini T.J.
Bueno P.C.P.
Cavalheiro A.J.
Hyslop S.
Puebla P.
Feliciano A.S.
Oshima-Franco Y.
Abstract: Of the various biological activities ascribed to extracts from Casearia sylvestris (guaçatonga), its facilitatory activity, i.e., ability to increase skeletal muscle contractile amplitude, has promising therapeutic applications. In this work, we investigated the components responsible for the previously described neurofacilitation caused by C. sylvestris leaves. Methods: The methanolic fraction of C. sylvestris leaves was initially fractionated by column chromatography and partitioned in a MeOH:H2O gradient. The resulting fractions were analyzed by analytical HPLC and yielded fraction 5:5 (F55) that was subjected to solid phase extraction and preparative HPLC. Of the seven resulting subfractions, only F55-6 caused muscle facilitation. Subfractions F55-6 and F55-7 (similar in composition to F55-6 by TLC analysis, but inactive) were analyzed by 1H-NMR to identify their constituents. Results: This analysis identified a rutin-glycoside phytocomplex that caused neurofacilitation, a property that commercial rutin alone did not exhibit. Conclusion: F55-6 apparently caused neurofacilitation by the same mechanism (presynaptic action) as the methanolic fraction since its activity was also inhibited in tetrodotoxin-pretreated preparations. © 2016 Bentham Science Publishers.
Subject: 1h-nmr
Chromatographic Techniques
Facilitatory Effect
Flavonoid Glycosides
Guaçatonga
Neuromuscular Junction
Editor: Bentham Science Publishers B.V.
Rights: fechado
Identifier DOI: 10.2174/1389201017666161117145947
Address: https://www.scopus.com/inward/record.uri?eid=2-s2.0-85009223503&doi=10.2174%2f1389201017666161117145947&partnerID=40&md5=d241926396f1cb2c4dbb972fca0f43a0
Date Issue: 2016
Appears in Collections:Unicamp - Artigos e Outros Documentos

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