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|Title:||Detection Of Pneumocystis Jirovecii By Nested Pcr In Hiv-negative Patients With Pulmonary Disease|
|Title Alternative:||Detección De Pneumocystis Jirovecii Mediante Pcr Anidada En Pacientes Vih Negativos Con Enfermedad Pulmonar|
de Assis Â.M.
|Abstract:||Nested PCR can be used to determine the status of Pneumocystis jirovecii infection in other lung diseases. Aims This study sought to detect a target DNA fragment (mitochondrial large subunit rRNA or mtL SUrRNA) of P. jirovecii in patients with lung disease who underwent bronchoscopy with collection of bronchoalveolar lavage (BAL). Methods The results from toluidine blue staining were compared with those obtained using molecular methods that included an “in house” DNA extraction procedure, PCR and nested PCR. Results Fifty-five BAL samples from patients with atypical chest X-rays were screened for P. jirovecii. None of the samples was positive for P. jirovecii using toluidine blue staining. In contrast, P. jirovecii DNA was detected by nested PCR in BAL samples from 36 of 55 patients (65.5%). The lung diseases in the patients included cancer, pneumonia, tuberculosis, and chronic obstructive pulmonary disease (COPD). Other chronic problems in the patients included hypertension, diabetes, smoking, and alcoholism. Conclusions Nested PCR showed high sensitivity for detecting P. jirovecii, especially when compared with toluidine blue staining. Using this method, P. jirovecii infection was detected in HIV-negative patients with lung disease. © 2016 Asociación Española de Micología|
|Editor:||Asociacion Espanola de Micologia|
|Appears in Collections:||Unicamp - Artigos e Outros Documentos|
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