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dc.typeArtigo de Periódicopt_BR
dc.titleIn Vitro Study Of The Antibacterial Properties And Impact Strength Of Dental Acrylic Resins Modified With A Nanomaterialpt_BR
dc.contributor.authorde Castropt_BR
dc.contributor.authorDT; Valentept_BR
dc.contributor.authorMLC; Agnellipt_BR
dc.contributor.authorJAM; da Silvapt_BR
dc.contributor.authorCHL; Watanabept_BR
dc.contributor.authorE; Siqueirapt_BR
dc.contributor.authorRL; Alvespt_BR
dc.contributor.authorOL; Holtzpt_BR
dc.contributor.authorRD; dos Reispt_BR
dc.description.abstractStatement of problem. The accumulation of bacteria on the surface of dental prostheses can lead to systemic disease. Purpose. The purpose of this in vitro study was to evaluate the growth of Staphylococcus aureus and Pseudomonas aeruginosa on the surface of autopolymerizing (AP) and heat -polymerizing (HP) acrylic resins incorporated with nanostructured silver vanadate (beta-AgVO3) and its impact strength. Material and methods. For each resin, 216 circular specimens (9x2 mm) were prepared for microbiologic analysis and 60 rectangular specimens (65x10x3.3 mm) for mechanical analysis, according to the percentage of beta-AgVO3: 0%, control group; 0.5%; 1%; 2.5%; 5%; and 10%. After a biofilm had formed, the metabolic activity of the bacteria was measured using the XTT reduction assay (2,3-bis-(2-methoxy-4-nitro-5-sulfophenyl)-2H-tetrazolium-5-carboxanilide) (n=8), and the number of viable cells was determined by counting colony forming units per milliliter (CFU/mL) (n=8). Confocal laser scanning microscopy (CLSM) was used to complement the analyses (n=2). The mechanical behavior was evaluated by impact strength assays (n=10). Data were analyzed by 2-way ANOVA, followed by the Tukey honestly significant difference (HSD) post hoc test (alpha=.05). Results. The addition of 5% and 10% beta-AgVO3 significantly decreased the metabolic activity of P. aeruginosa for both resins (P<.05). The HP resin promoted a greater reduction in metabolic activity than the AP resin (P<.05). No difference was found in the metabolic activity of S. aureus according to the XTT (P>.05). The number of CFU/mL for S. aureus and P. aeruginosa decreased significantly when 5% and 10% beta-AgVO3 were added (P<.001). These concentrations significantly reduced the impact strength of the resins (P<.001) because the system was weakened by the presence of clusters of beta-AgVO3. Conclusion. The addition of beta-AgVO3 can provide acrylic resins with antibacterial activity but reduces their impact strength. More efficient addition methods should be investigated.en
dc.relation.ispartofJournal Of Prosthetic Dentistrypt_BR
dc.publisher.cityNEW YORKpt_BR
dc.identifier.citationJournal Of Prosthetic Dentistry. MOSBY-ELSEVIER, n. 115, n. 2, p. 238 - 246.pt_BR
dc.description.sponsorshipFoundation for Research Support of the State of Sao Paulo [FAPESP 2012/09124-1, 2012/09347-0]pt_BR
dc.description.sponsorship1Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)pt_BR
dc.description.provenanceMade available in DSpace on 2016-12-06T18:33:19Z (GMT). No. of bitstreams: 1 000369467400018.pdf: 4843300 bytes, checksum: 0f59c5ec4c6fdc5e5d124429542c622b (MD5) Previous issue date: 2016en
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