Please use this identifier to cite or link to this item: http://repositorio.unicamp.br/jspui/handle/REPOSIP/320522
Type: Artigo de Periódico
Title: Excessive Training Impairs The Insulin Signal Transduction In Mice Skeletal Muscles
Author: Pereira
BC; da Rocha
AL; Pinto
AP; Pauli
JR; de Moura
LP; Mekary
RA; de Freitas
EC; da Silva
ASR
Abstract: The main aim of this investigation was to verify the effects of overtraining (OT) on the insulin and inflammatory signaling pathways in mice skeletal muscles. Rodents were divided into control (CT), overtrained by downhill running (OTR/down), overtrained by uphill running (OTR/up), and overtrained by running without inclination (OTR) groups. Rotarod, incremental load, exhaustive, and grip force tests were used to evaluate performance. Thirty-six hours after the grip force test, the extensor digitorum longus (EDL) and soleus were extracted for subsequent protein analyses. The three OT protocols led to similar responses of all performance evaluation tests. The phosphorylation of insulin receptor beta (pIR beta; Tyr), protein kinase B (pAkt; Ser473), and the protein levels of plasma membrane glucose transporter-4 (GLUT4) were lower in the EDL and soleus after the OTR/down protocol and in the soleus after the OTR/up and OTR protocols. While the pIR beta was lower after the OTR/up and OTR protocols, the pAkt was higher after the OTR/up in the EDL. The phosphorylation of I kappa B kinase alpha and beta (pIKK alpha/beta; Ser180/181), stress-activated protein kinases/Jun amino-terminal kinases (pSAPK-JNK; Thr183/Tyr185), factor nuclear kappa B (pNF kappa B p65; Ser536), and insulin receptor substrate 1 (pIRS1; Ser307) were higher after the OTR/down protocol, but were not altered after the two other OT protocols. In summary, these data suggest that OT may lead to skeletal muscle insulin signaling pathway impairment, regardless of the predominance of eccentric contractions, although the insulin signal pathway impairment induced in OTR/up and OTR appeared to be muscle fiber-type specific.
Subject: Exercise
Insulin Signaling
Plasma Membrane Glut4
Mouse
Editor: BIOSCIENTIFICA LTD
Citation: Journal Of Endocrinology. BIOSCIENTIFICA LTD, n. 230, n. 1, p. 93 - 104.
Rights: fechado
Identifier DOI: 10.1530/JOE-16-0063
Address: http://joe.endocrinology-journals.org/content/230/1/93.abstract?sid=dc4f7156-b33f-4cb0-b5da-6e83fbfbc86b
Date Issue: 2016
Appears in Collections:Unicamp - Artigos e Outros Documentos

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