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dc.contributor.CRUESPUNIVERSIDADE DE ESTADUAL DE CAMPINASpt_BR
dc.typeArtigo de Periódicopt_BR
dc.titlePhylogenetic And Molecular Variability Studies Reveal A New Genetic Clade Of Citrus Leprosis Virus Cpt_BR
dc.contributor.authorRamos-Gonzalezpt_BR
dc.contributor.authorPL; Chabi-Jesuspt_BR
dc.contributor.authorC; Guerra-Perazapt_BR
dc.contributor.authorO; Bretonpt_BR
dc.contributor.authorMC; Arenapt_BR
dc.contributor.authorGD; Nunespt_BR
dc.contributor.authorMA; Kitajimapt_BR
dc.contributor.authorEW; Machadopt_BR
dc.contributor.authorMA; Freitas-Astuapt_BR
dc.contributor.authorJpt_BR
unicamp.author.emailplrg1970@gmail.com; millachabi@yahoo.com.br; onelio@protonmail.com; mbreton@uol.com.br; gaby.arena@gmail.com; mandreian@yahoo.com; ewkitaji@usp.br; marcos@centrodecitricultura.br; juliana.astua@embrapa.brpt_BR
dc.subjectCileviruspt_BR
dc.subjectCitrus Leprosispt_BR
dc.subjectBrevipalpus-transmitted Virusespt_BR
dc.description.abstractCitrus leprosis virus C (CiLV-C) causes a severe disease affecting citrus orchards in the Western hemisphere. This study reveals the molecular variability of the virus by analyzing four genomic regions (p29, p15, MP and RNA2-intergenic region) distributed over its two RNAs. Nucleotide diversity (pi) values were relatively low but statistically different over the analyzed genes and subpopulations, indicating their distinct evolutionary history. Values of pi(p29) and pi(MP) were higher than those of pi(p15) and pi(RNA2-IR), whereas pi(MP) was increased due to novel discovered isolates phylogenetically clustered in a divergent clade that we called SJP. Isolate BR_SP_SJP_01 RNA1 and RNA2 sequences, clade SJP, showed an identity of 85.6% and 88.4%, respectively, with those corresponding to CiLV-C, the type member of the genus Cilevirus, and its RNA2 5'-proximal region was revealed as a minor donor in a putative inter-clade recombination event. In addition to citrus, BR_SP_SJP_01 naturally infects the weed Commelina benghalensis and is efficiently transmitted by Brevipalpus yothersi mites. Our data demonstrated that negative selection was the major force operating in the evaluated viral coding regions and defined amino acids putatively relevant for the biological function of cilevirus proteins. This work provides molecular tools and sets up a framework for further epidemiological studies.en
dc.relation.ispartofViruses-Baselpt_BR
dc.publisher.cityBASELpt_BR
dc.publisherMDPI AGpt_BR
dc.date.issued2016pt_BR
dc.identifier.citationViruses-basel. MDPI AG, n. 8, n. 6, p. .pt_BR
dc.language.isoEnglishpt_BR
dc.description.volume8pt_BR
dc.description.issuenumberpt_BR
dc.description.lastpagept_BR
dc.rightsabertopt_BR
dc.sourceWOSpt_BR
dc.identifier.issn1999-4915pt_BR
dc.identifier.wosidWOS:000378848600005pt_BR
dc.identifier.doi10.3390/v8060153pt_BR
dc.identifier.urlhttp://www.mdpi.com/1999-4915/8/6/153pt_BR
dc.description.sponsorshipConselho Nacional de Desenvolvimento Cientifico e Tecnologico [375843/2012-4, 481771/2013-1, 401564/2012-6]pt_BR
dc.description.sponsorshipFundacao de Apoio a Pesquisa no Estado de Sao Paulo [2012/18771-0, 2012/20667-7, 2014/08458-9]pt_BR
dc.description.sponsorship1Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)pt_BR
dc.date.available2016-12-06T18:32:22Z-
dc.date.accessioned2016-12-06T18:32:22Z-
dc.description.provenanceMade available in DSpace on 2016-12-06T18:32:22Z (GMT). No. of bitstreams: 1 000378848600005.pdf: 6940821 bytes, checksum: e62319c80855bc3cba195dd1e8625c58 (MD5) Previous issue date: 2016en
dc.identifier.urihttp://repositorio.unicamp.br/jspui/handle/REPOSIP/320520-
dc.description.conferencelocationpt_BR
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