Please use this identifier to cite or link to this item: http://repositorio.unicamp.br/jspui/handle/REPOSIP/320466
Type: Artigo de Periódico
Title: Global Proteome Profiling Of Dental Cementum Under Experimentally-induced Apposition
Author: Salmon
CR; Giorgetti
APO; Leme
AFP; Domingues
RR; Sallum
EA; Alves
MC; Kolli
TN; Foster
BL; Nociti
FH
Abstract: Dental cementum (DC) covers the tooth root and has important functions in tooth attachment and position. DC can be lost to disease, and regeneration is currently unpredictable due to limited understanding of DC formation. This study used a model of experimentally-induced apposition (EIA) in mice to identify proteins associated with new DC formation. Mandibular first molars were induced to super-erupt for 6 and 21 days after extracting opposing maxillary molars. Decalcified and formalin-fixed paraffin-embedded mandible sections were prepared for laser capture microdissection. Microdissected protein extracts were analyzed by liquid chromatography coupled to tandem mass spectrometry (LC-MS/MS), and the data submitted to repeated measure ANOVA test (RM-ANOVA, alpha = 5%). A total of 519 proteins were identified, with 97 (18.6%) proteins found exclusively in EIA sites and 50 (9.6%) proteins exclusively expressed in control sites. Fifty six (10.7%) proteins were differentially regulated by RM-ANOVA (p < 0.05), with 24 regulated by the exclusive effect of EIA (12 proteins) or the interaction between EIA and time (12 proteins), including serpin la, procollagen C-endopeptidase enhancer, tenascin X (TNX), and asporin (ASPN). In conclusion, proteomic analysis demonstrated significantly altered protein profile in DC under EIA, providing new insights on DC biology and potential candidates for tissue engineering applications. Significance: Dental cementum (DC) is a mineralized tissue that covers the tooth root surface and has important functions in tooth attachment and position. DC and other periodontal tissues can be lost to disease, and regeneration is currently unpredictable due to lack of understanding of DC formation. This study used a model of experimentally-induced apposition (EIA) in mice to promote new cementum formation, followed by laser capture microdissection (LCM) and liquid chromatography coupled to tandem mass spectrometry (LC-MS/MS) proteomic analysis. This approach identified proteins associated with new cementum formation that may be targets for promoting cementum regeneration. (C) 2016 Elsevier B.V. All rights reserved.
Subject: Dental Cementum
Proteomic Analysis
Periodontal Ligament
Tissue Apposition
Editor: ELSEVIER SCIENCE BV
Citation: Journal Of Proteomics. ELSEVIER SCIENCE BV, n. 141, p. 12 - 23.
Rights: fechado
Identifier DOI: 10.1016/j.jprot2016.03.036
Address: http://www-sciencedirect-com.ez88.periodicos.capes.gov.br/science/article/pii/S1874391916300987
Date Issue: 2016
Appears in Collections:Unicamp - Artigos e Outros Documentos

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