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Type: Artigo de Periódico
Title: 15-deoxy-delta(12,14)-prostaglandin J(2) Induces Apoptosis And Upregulates Socs3 In Human Thyroid Cancer Cells
Author: Trindade-da-Silva
CA; Reis
CF; Vecchi
L; Napimoga
MH; Sperandio
M; Colombo
BFM; Alves
PT; Ward
LS; Ueira-Vieira
C; Goulart
Abstract: The cyclopentenone prostaglandin 15-deoxy-Delta(12,14)-prostaglandin J(2) (15d-PGJ(2)) is a natural ligand of peroxisome proliferator-activated receptor gamma (PPAR-gamma) and a potential mediator of apoptosis in cancer cells. In the present study, we evaluated the effect of 15d-PGJ(2) in human thyroid papillary carcinoma cells (TPC-1) using different doses of 15d-PGJ(2) (0.6 to 20 mu M) to determine IC50 (9.3 mu M) via the MTT assay. The supernatant culture medium of the TPC-1 cells that was treated either with 15d-PGJ(2) or with vehicle (control) for 24 hours was assessed for IL-6 secretion via CBA assay. RT-qPCR was used to evaluate mRNA expression of IL-6, SOCS1, SOCS3, and STAT3. TPC-1 cells treated with 15d-PGJ(2) decreased the secretion and expression of IL-6 and STAT3, while it increased SOCS1 and SOCS3. Overall, we demonstrated that 15d-PGJ(2) downregulated IL-6 signaling pathway and led TPC-1 cells into apoptosis. In conclusion, 15d-PGJ(2) shows the potential to become a new therapeutic approach for thyroid tumors.
Subject: Activated-receptor-gamma
Il-6 Production
Lineage Cells
Citation: Ppar Research . HINDAWI PUBLISHING CORP, n. 4106297, p. .
Rights: aberto
Identifier DOI: 10.1155/2016/4106297
Date Issue: 2016
Appears in Collections:Unicamp - Artigos e Outros Documentos

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