Please use this identifier to cite or link to this item: http://repositorio.unicamp.br/jspui/handle/REPOSIP/320401
Type: Artigo de Periódico
Title: An Evaluation Of The Crystal Structure Of C-terminal Truncated Apolipoprotein A-i In Solution Reveals Structural Dynamics Related To Lipid Binding
Author: Melchior
JT; Walker
RG; Morris
J; Jones
MK; Segrest
JP; Lima
DB; Carvalho
PC; Gozzo
FC; Castleberry
M; Thompson
TB; Davidson
WS
Abstract: Apolipoprotein (apo) A-I mediates many of the anti-atherogenic functions attributed to high density lipoprotein. Unfortunately, efforts toward a high resolution structure of full-length apoA-I have not been fruitful, although there have been successes with deletion mutants. Recently, a C-terminal truncation (apoA-I185-243) was crystallized as a dimer. The structure showed two helical bundles connected by a long, curved pair of swapped helical domains. To compare this structure to that existing under solution conditions, we applied small angle x-ray scattering and isotope-assisted chemical cross-linking to apoA-I185-243 in its dimeric and monomeric forms. For the dimer, we found evidence for the shared domains and aspects of the N-terminal bundles, but not the molecular curvature seen in the crystal. We also found that the N-terminal bundles equilibrate between open and closed states. Interestingly, this movement is one of the transitions proposed during lipid binding. The monomer was consistent with a model in which the long shared helix doubles back onto the helical bundle. Combined with the crystal structure, these data offer an important starting point to understand the molecular details of high density lipoprotein biogenesis.
Subject: Apolipoprotein
Mass Spectrometry (ms)
Oligomerization
Small-angle X-ray Scattering (saxs)
Structural Biology
Structural Model
Editor: AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC
Citation: Journal Of Biological Chemistry. AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC, n. 291, n. 10, p. 5439 - 5451.
Rights: fechado
Identifier DOI: 10.1074/jbc.M115.706093
Address: http://www-jbc-org.ez88.periodicos.capes.gov.br/content/291/10/5439
Date Issue: 2016
Appears in Collections:Unicamp - Artigos e Outros Documentos

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