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Type: Artigo de Periódico
Title: Co-cultivation Of Aspergillus Nidulans Recombinant Strains Produces An Enzymatic Cocktail As Alternative To Alkaline Sugarcane Bagasse Pretreatment
Author: Lima
MS; Damasio
ARD; Crnkovic
PM; Pinto
MR; da Silva
AM; da Silva
JR; Segato
F; de Lucas
RC; Jorge
JA; Polizeli
Abstract: Plant materials represent a strategic energy source because they can give rise to sustainable biofuels through the fermentation of their carbohydrates. A clear example of a plant-derived biofuel resource is the sugar cane bagasse exhibiting 60-80% of fermentable sugars in its composition. However, the current methods of plant bioconversion employ severe and harmful chemical/physical pretreatments raising biofuel cost production and environmental degradation. Replacing these methods with co-cultivated enzymatic cocktails is an alternative. Here we propose a pretreatment for sugarcane bagasse using a multi-enzymatic cocktail from the co-cultivation of four Aspergillus nidulans recombinant strains. The co-cultivation resulted in the simultaneous production of GH51 arabinofuranosidase (AbfA), GH11 endo-1,4-xylanase (XInA), GH43 endo-1,5-arabinanase (AbnA) and GH12 xyloglucan specific endo-beta-1,4-glucanase (XegA). This core set of recombinant enzymes was more efficient than the alternative alkaline method in maintaining the cellulose integrity and exposing this cellulose to the following saccharification process. Thermogravimetric and differential thermal analysis revealed residual byproducts on the alkali pretreated biomass, which were not found in the enzymatic pretreatment. Therefore, the enzymatic pretreatment was residue-free and seemed to be more efficient than the applied alkaline method, which makes it suitable for bioethanol production.
Subject: Aspergillus Nidulans
Thermogravimetric Analysis
Differential Thermal Analysis
Naoh Pretreatment
Enzymatic Pretreatment
Sugarcane Bagasse
Enzymatic Cocktail
Citation: Frontiers In Microbiology. FRONTIERS MEDIA SA, n. 7, n. 683, p. .
Rights: aberto
Identifier DOI: 10.3389/fmicb.2016.00583
Date Issue: 2016
Appears in Collections:Unicamp - Artigos e Outros Documentos

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