Please use this identifier to cite or link to this item: http://repositorio.unicamp.br/jspui/handle/REPOSIP/320262
Type: Artigo de Periódico
Title: Exploiting The Biological Roles Of The Trypsin Inhibitor From Inga Vera Seeds: A Multifunctional Kunitz Inhibitor
Author: Bezerra
CD; de Oliveira
CFR; Machado
OLT; de Mello
GSV; Pitta
MGD; Rego
MJBD; Napoleao
TH; Paiva
PMG; Ribeiro
SDF; Gomes
VM; Silva
ON; Maria-Neto
S; Franco
OL; Macedo
MLR
Abstract: Here, the purification, biochemical and biological properties of a trypsin inhibitor from Ingot vera seeds (IVTI) are described. Partial amino acid sequence of IVTI showed that it belongs to the MEROPS 103 Kunitz inhibitor family. Moreover, it is composed of a single 20 kDa polypeptide chain with one disulfide bridge and was capable of inhibiting bovine trypsin at a 1:1 molar ratio with a 1.15 nM inhibition constant. IVTI was stable over a wide range of temperature, pH and concentration of a reducing agent. IVTI also inhibited the trypsin-like enzymes from the midgut of lepidopteran pests, such as Anagasta kuehniella (89%), Spodoptera frugiperda (83%), Corcyra cephalonica (80%), Heliothis virescens (70%) and Helicoverpa zea (64%). Furthermore, bioinsecticidal assays against A. kuehniella demonstrated that IVTI affected larval development by impairing weight gain and survival, as well as altering the duration of the larval cycle. IVTI was also fungicidal to Candida buinensis and bacteriostatic agent to Escherichia coli. Further assays revealed that IVTI is a chemopreventive agent against human epithelial colorectal adenocarcinoma cells (CACO-2), reducing cell viability by 70% at 200 mu g mL(-1). In summary, these results demonstrate the multifaceted potential of IVTI as a biotechnological tool for agriculture and healthcare. (C) 2016 Elsevier Ltd. All rights reserved.
Subject: Inga Vera
Plant-defense
Kunitz Inhibitor
Antimicrobial
Anticancer
Bioinsecticidal
Editor: ELSEVIER SCI LTD
Rights: fechado
Identifier DOI: 10.1016/j.procbio.2016.03.008
Address: http://www-sciencedirect-com.ez88.periodicos.capes.gov.br/science/article/pii/S1359511316300381
Date Issue: 2016
Appears in Collections:Unicamp - Artigos e Outros Documentos

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