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Type: Artigo de Periódico
Title: Effects Of Low Level Laser Therapy On Inflammatory And Angiogenic Gene Expression During The Process Of Bone Healing: A Microarray Analysis
Author: Tim
CR; Bossini
PS; Kido
HW; Malavazi
I; Kress
MRV; Carazzolle
MF; Parizotto
NA; Renno
Abstract: The process of bone healing as well as the expression of inflammatory and angiogenic genes after low level laser therapy (LLLT) were investigated in an experimental model of bone defects. Sixty Wistar rats were distributed into control group and laser group (830 nm, 30 mW, 2,8 J, 94 seg). Histopathological analysis showed that LLLT was able to modulate the inflammatory process in the area of the bone defect and also to produce an earlier deposition of granulation tissue and newly formed bone tissue. Microarray analysis demonstrated that LLLT produced an up-regulation of the genes related to the inflammatory process (MMD, PTGIR, PTGS2, Ptger2, ILL 1IL6, IL8, IL18) and the angiogenic genes (FGF14, FGF2, ANGPT2, ANGPT4 and PDGFD) at 36 h and 3 days, followed by the decrease of the gene expression on day 7. Immunohistochemical analysis revealed that the subjects that were treated presented a higher expression of COX-2 at 36 h after surgery and an increased VEGF expression on days 3 and 7 after surgery. Our findings indicate that LLLT was efficient on accelerating the development of newly formed bone probably by modulating the inflammatory and angiogenic gene expression as well as COX2 and VEGF immunoexpression during the initial phase of bone healing. (C) 2015 Elsevier B.V. All rights reserved.
Subject: Bone Repair
Gene Expression
Citation: Journal Of Photochemistry And Photobiology B: Biology. ELSEVIER SCIENCE SA, n. 154, p. 8 - 15.
Rights: fechado
Identifier DOI: 10.1016/j.jphotobiol.2015.10.028
Date Issue: 2016
Appears in Collections:Unicamp - Artigos e Outros Documentos

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