Please use this identifier to cite or link to this item:
Type: Artigo de Periódico
Title: The Involvement Of Clathrin-mediated Endocytosis And Two Sid-1-like Transmembrane Proteins In Doublestranded Rna Uptake In The Colorado Potato Beetle Midgut
Author: Cappelle
K; de Oliveira
CFR; Van Eynde
B; Christiaens
O; Smagghe
Abstract: RNA interference (RNAi) is a powerful tool in entomology and shows promise as a crop protection strategy, but variability in its efficiency across different insect species limits its applicability. For oral uptake of the double-stranded RNA (dsRNA), the RNAi trigger, two different mechanisms are known: systemic RNA interference deficient-1 (Sid-1) transmembrane channel-mediated uptake and clathrin-mediated endocytosis. So far, a wide range of experiments has been conducted, confirming the involvement of one of the pathways in dsRNA uptake, but never both pathways in the same species. We investigated the role of both pathways in dsRNA uptake in the Colorado potato beetle, Leptinotarsa decemlineata, known to have an efficient RNAi response. Through RNAi-of-RNAi experiments, we demonstrated the contribution of two different sid-1-like (sil) genes, silA and silC, and clathrin heavy chain and the 16kDa subunit of the vacuolar H 1 ATPase (vha16), elements of the endocytic pathway, to the RNAi response. Furthermore, the sid-1-like genes were examined through phylogenetic and hydrophobicity analysis. This article reports for the first time on the involvement of two pathways in dsRNA uptake in an insect species and stresses the importance of evaluating both pathways through a well-devised reporter system in any future experiments on cellular dsRNA uptake.
Subject: Colorado Potato Beetle
Rna Interference
Double-stranded Rna Uptake
Systemic Rna Interference Deficient-1 (sid-1)
Citation: Insect Molecular Biology. WILEY-BLACKWELL, n. 25, n. 3, p. 315 - 323.
Rights: fechado
Identifier DOI: 10.1111/imb.12222
Date Issue: 2016
Appears in Collections:Unicamp - Artigos e Outros Documentos

Files in This Item:
File SizeFormat 
000375669800011.pdf298.26 kBAdobe PDFView/Open

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.