Please use this identifier to cite or link to this item: http://repositorio.unicamp.br/jspui/handle/REPOSIP/320125
Type: Artigo de Periódico
Title: Dietary Interesterified Fat Enriched With Palmitic Acid Induces Atherosclerosis By Impairing Macrophage Cholesterol Efflux And Eliciting Inflammation
Author: Afonso
MS; Lavrador
MSF; Koike
MK; Cintra
DE; Ferreira
FD; Nunes
VS; Castilho
G; Gioielli
LA; Bombo
RP; Catanozi
S; Caldini
EG; Damaceno-Rodrigues
NR; Passarelli
M; Nakandakare
ER; Lottenberg
AM
Abstract: Interesterified fats are currently being used to replace trans fatty acids. However, their impact on biological pathways involved in the atherosclerosis development was not investigated. Weaning male LDLr-KO mice were fed for 16 weeks on a high-fat diet (40% energy as fat) containing polyunsaturated (PUFA), TRANS, palmitic (PALM), palmitic interesterified (PALM INTER), stearic (STEAR) or stearic interesterified (STEAR INTER). Plasma lipids, lipoprotein profile, arterial lesion area, macrophage infiltration, collagen content and inflammatory response modulation were determined. Macrophage cholesterol efflux and the arterial expression of cholesterol uptake and efflux receptors were also performed. The interesterification process did not alter plasma lipid concentrations. Although PALM INTER did not increase plasma cholesterol concentration as much as TRANS, the cholesterol enrichment in the LDL particle was similar in both groups. Moreover, PALM INTER induced the highest IL-1 beta, MCP-1 and IL-6 secretion from peritoneal macrophages as compared to others. This inflammatory response elicited by PALM INTER was confirmed in arterial wall, as compared to PALM. These deleterious effects of PALM INTER culminate in higher atherosclerotic lesion, macrophage infiltration and collagen content than PALM, STEAR, STEAR INTER and PUFA. These events can partially be attributed to a macrophage cholesterol accumulation, promoted by apoAl and HDL2-mediated cholesterol efflux impairment and increased Olr-1 and decreased Abca1 and Nr1h3 expressions in the arterial wall. Interesterified fats containing palmitic acid induce atherosclerosis development by promoting cholesterol accumulation in LDL particles and macrophagic cells, activating the inflammatory process in LDLr-KO mice. (C) 2016 Elsevier Inc. All rights reserved.
Subject: Atherosclerosis
Interesterified Fat
Trans Fat
Diet
Lipoprotein Metabolism
Mice
Editor: ELSEVIER SCIENCE INC
Citation: The Journal Of Nutritional Biochemistry. ELSEVIER SCIENCE INC, n. 32, p. 91 - 100.
Rights: fechado
Identifier DOI: 10.1016/j.jnutbio.2016.01.005
Address: http://www-sciencedirect-com.ez88.periodicos.capes.gov.br/science/article/pii/S095528631600036X
Date Issue: 2016
Appears in Collections:Unicamp - Artigos e Outros Documentos

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