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Type: Artigo de Periódico
Title: Reduced Insulin Secretion Function Is Associated With Pancreatic Islet Redistribution Of Cell Adhesion Molecules (cams) In Diabetic Mice After Prolonged High-fat Diet
Author: Falcao
VTFL; Maschio
DA; de Fontes
CC; Oliveira
RB; Santos-Silva
JC; Almeida
ACS; Vanzela
EC; Cartaxo
MT; Carvalho
CPF; Collares-Buzato
Abstract: Intercellular junctions play a role in regulating islet cytoarchitecture, insulin biosynthesis and secretion. In this study, we investigated the animal metabolic state as well as islet histology and cellular distribution/expression of CAMs and F-actin in the endocrine pancreas of C57BL/6/JUnib mice fed a high-fat diet (HFd) for a prolonged time period (8 months). Mice fed a HFd became obese and type 2 diabetic, displaying significant peripheral insulin resistance, hyperglycemia and moderate hyperinsulinemia. Isolated islets of HFd-fed mice displayed a significant impairment of glucose-induced insulin secretion associated with a diminished frequency of intracellular calcium oscillations compared with control islets. No marked change in islet morphology and cytoarchitecture was observed; however, HFd-fed mice showed higher beta cell relative area in comparison with controls. As shown by immunohistochemistry, ZO-1, E-, N-cadherins, alpha- and beta-catenins were expressed at the intercellular contact site of endocrine cells, while VE-cadherin, as well as ZO-1, was found at islet vascular compartment. Redistribution of N-, E-cadherins and alpha-catenin (from the contact region to the cytoplasm in endocrine cells) associated with increased submembranous F-actin cell level as well as increased VE-cadherin islet immunolabeling was observed in diabetic mice. Increased gene expression of VE-cadherin and ZO-1, but no change for the other proteins, was observed in islets of diabetic mice. Only in the case of VE-cadherin, a significant increase in islet content of this CAM was detected by immunoblotting in diabetic mice. In conclusion, CAMs are expressed by endocrine and endothelial cells of pancreatic islets. The distribution/expression of N-, E- and VE-cadherins as well as alpha-catenin and F-actin is significantly altered in islet cells of obese and diabetic mice.
Subject: Cell Adhesion Molecules
Cell-cell Interactions
Pancreatic Beta Cells
Type 2 Diabetes Mellitus
High-fat Diet
Insulin Secretion
Citation: Histochemistry And Cell Biology. SPRINGER, n. 146, n. 1, p. 13 - 31.
Rights: fechado
Identifier DOI: 10.1007/s00418-016-1428-5
Date Issue: 2016
Appears in Collections:Unicamp - Artigos e Outros Documentos

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