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Type: Artigo de Periódico
Title: Effects Of Fish Oil Containing Eicosapentaenoic Acid And Docosahexaenoic Acid On Dystrophic Mdx Mice Hearts At Later Stages Of Dystrophy
Author: Mauricio
AF; Pereira
JA; Neto
HS; Marques
Abstract: In the present study, we investigated whether omega-3 would be effective against dystrophic cardiomyopathy at later stages (13 and 17 mo) of the disease. Methods: Mdx mice (8 mo old) received omega-3 oil (commercially available fish oil; FDC vitamins; omega-3) for 5 mo. Untreated-mdx mice received mineral oil. Heart and diaphragm muscle were evaluated by morphometric (fibrosis), molecular (western blot, inflammatory markers), biochemical (creatine kinase), and functional (electrocardiogram) analyses. Results: Mdx mice presented elevated plasma levels of cardiac creatine kinase (41.2 U/L in normal x 119.6 U/L in untreated-mdx mice), which were significantly decreased by omega-3 treatment. Heart fibrosis was significantly ameliorated by omega-3 treatment at 17 mo of age (untreated-mdx: 20.8% of fibrosis; omega-3-treated: 15.7% of fibrosis in right ventricle). Omega-3 improved some electrocardiogram parameters. Markers of inflammation (tumor necrosis factor alpha, matrix metalloprotease-9, and tissue inhibitor of metalloprotease 1) in mdx heart were significantly decreased by omega-3 treatment. Omega-3 increased beta-dystroglycan levels in mdx heart and did not affect the levels of the profibrotic transforming growth factor beta. Omega-3 ameliorated the dystrophic diaphragm in almost all of the parameters evaluated (fibrosis, transforming growth factor beta, metalloprotease-9, and tissue inhibitor of metalloprotease 1). Conclusions: The present study suggests that omega-3 may be useful in ameliorating dystrophic cardiomyopathy and diaphragm dystrophy in mdx mice at later stages of the disease, further supporting the use of omega-3 in DMD clinical trials. (C) 2016 Elsevier Inc. All rights reserved.
Subject: Cardiomyopathy
Citation: Nutrition. ELSEVIER SCIENCE INC, n. 32, n. 7-8, p. 855 - 862.
Rights: fechado
Identifier DOI: 10.1016/j.nut.2016.01.015
Date Issue: 2016
Appears in Collections:Unicamp - Artigos e Outros Documentos

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