Please use this identifier to cite or link to this item: http://repositorio.unicamp.br/jspui/handle/REPOSIP/319951
Type: Resenha
Title: Laser Ablation And Inductively Coupled Plasma Mass Spectrometry Focusing On Bioimaging From Elemental Distribution Using Matlab Software: A Practical Guide
Author: Pessoa
GD; Capelo-Martinez
JL; Fdez-Riverola
F; Lopez-Fernandez
H; Glez-Pena
D; Reboiro-Jato
M; Arruda
MAZ
Abstract: The parameters influencing laser ablation inductively coupled plasma mass spectrometry as a tool for imaging elemental distribution in tissues are critically commented on in this work, and penile cancer tissue was used as a model. General aspects of LA-ICP-MS are discussed, and among them, issues regarding sample preparation and calibration. The optimization process of the following variables is described in detail in a step-by-step tutorial manner: laser intensity, laser frequency, laser resolution, ICP radiofrequency, nebulizer and auxiliary gas flow rates, and C isotopes as internal standards. Once the best condition is found for each variable, MATLAB software is used to generate two-dimensional images. Herein it is also explained how to use MATLAB software to generate tissue images using acquisition, exporting and data treatment parameters.
Subject: La-icp-ms
Solution-based Calibration
Biological Samples
Trace-elements
Tissue
Strategies
Fixation
Section
Metals
Zinc
Editor: ROYAL SOC CHEMISTRY
Citation: Journal Of Analytical Atomic Spectrometry. ROYAL SOC CHEMISTRY, n. 31, n. 4, p. 832 - 840.
Rights: fechado
Identifier DOI: 10.1039/c5ja00451a
Address: http://pubs.rsc.org/en/Content/ArticleLanding/2016/JA/c5ja00451a#!divAbstract
Date Issue: 2016
Appears in Collections:Unicamp - Artigos e Outros Documentos

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