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|Type:||Artigo de Periódico|
|Title:||Establishment Of A Method For Describing Stx Genes Expression Of E.coli O157:h7 In Ground Beef Matrix During Refrigerated Storage|
|Abstract:||Ingredients and complexity of food matrices are inhibitory factors for gene expression studies of bacterial pathogens directly from food matrix. The aim of this study was to develop a simple and fast method for RNA extraction from ground beef matrix as well as stx genes expression of Escherichia coliO157:H7 by relative quantitative real-time reverse transcription-polymerase chain reaction (RT-PCR). Inoculated ground beef samples were kept 7 days in refrigerated storage (41C) and bacterial RNA was extracted according to the described method at 0, 2, 5 and 7 days after storage. Results indicate that refrigerated storage temperature decreased gene expression of stx1A (-3.35 relative changes on day 7) and stx2A (-2.9 relative changes on day 7) in ground beef matrix. In the current study, a method requiring minimal equipment and limited interference for RNA extraction from bacteria associated with a meat matrix was evaluated (developed, invested, etc.). Results of the study suggest that the quantitative real-time RT-PCR method could be used as a reliable and sensitive method for gene expression studies.|
|Appears in Collections:||Unicamp - Artigos e Outros Documentos|
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