Please use this identifier to cite or link to this item: http://repositorio.unicamp.br/jspui/handle/REPOSIP/319773
Type: Artigo de Periódico
Title: Determination Of 17 Beta-estradiol And Noradrenaline In Dog Serum Using Surface-enhanced Raman Spectroscopy And Random Forest
Author: Albuquerque
CDL; Nogueira
RB; Poppi
RJ
Abstract: In this paper is,proposed a methodology for quantification of two veterinary hormones (17 beta-estradiol and noradrenaline) in dog serum based on surface-enhanced Raman spectroscopy (SERS) in conjunction with Random Forest for data treatment. It does not require sample pre-treatment and was automated using a multiwell plate holder from Raman equipment of 96 well plates, allowing the sequential analysis without manipulations in less than one hour. Comparable results with ELISA reference method were achieved, with relative errors of prediction not superior to 15% for 17 beta-estradiol and 7% for noradrenaline. Studies concerning calculations of systematic errors, tendency in the residuals, interval confidence and variable importance were performed permitting certification of the methodology validity and identification of SERS bands related to the hormones quantification. (C) 2016 Elsevier B.V. All rights reserved.
Subject: Dog Serum
Hormones
Sers
Random Forest
Variable Importance
Editor: ELSEVIER SCIENCE BV
Rights: fechado
Identifier DOI: 10.1016/j.microc.2016.04.012
Address: http://www-sciencedirect-com.ez88.periodicos.capes.gov.br/science/article/pii/S0026265X16300388
Date Issue: 2016
Appears in Collections:Unicamp - Artigos e Outros Documentos

Files in This Item:
File SizeFormat 
000379633800014.pdf1.03 MBAdobe PDFView/Open


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.