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Type: Artigo de Periódico
Title: Amperometric Quantification Of S-nitrosoglutathione Using Gold Nanoparticles: A Step Toward Determination Of S-nitrosothiols In Plasma
Author: Baldim
V; Ismail
A; Taladriz-Blanco
P; Griveau
S; de Oliveira
MG; Bedioui
Abstract: S-Nitrosothiols (RSNOs) are carriers of nitric oxide (NO) and have important biological activities. We propose here the use of gold nanoparticles (AuNPs) and NO-selective amperometric microsensor for the detection and quantification Of S-nitro-soglutathione (GSNO) as a step toward the determination of plasma RSNOs. AuNPs were used to decompose RSNOs with the quantitative release of free NO which was selectively detected, with a NO microsensor. The optimal [GSNO]/[AuNPs] ratio was determined, corresponding to an excess of AuNP surface relative to the molar GSNO amount. Moreover, the influence of free plasma thiols on this method was investigated and a protocol based on the blocking of free thiols, with iodoacetic acid, forming the carboxymethyl derivative of the cysteine residues, is proposed.
Citation: Analytical Chemistry. AMER CHEMICAL SOC, n. 88, n. 6, p. 3115 - 3120.
Rights: fechado
Identifier DOI: 10.1021/acs.analchem.5b04035
Date Issue: 2016
Appears in Collections:Unicamp - Artigos e Outros Documentos

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