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dc.contributor.CRUESPUNIVERSIDADE DE ESTADUAL DE CAMPINASpt_BR
dc.typeArtigo de periódicopt_BR
dc.titleBac-pool Sequencing And Assembly Of 19 Mb Of The Complex Sugarcane Genomept_BR
unicamp.author.emailparruda@unicamp.brpt_BR
unicamp.authorOkura, V.K., Centro de Biologia Molecular e Engenharia Genética, Universidade Estadual de Campinas, Campinas, Brazil, Laboratório Central de Tecnologias de Alto Desempenho em Ciências da Vida, Universidade Estadual de Campinas, Campinas, Brazilpt_BR
unicamp.authorde Souza, R.S.C., Centro de Biologia Molecular e Engenharia Genética, Universidade Estadual de Campinas, Campinas, Brazilpt_BR
unicamp.authorde Siqueira Tada, S.F., Laboratório Central de Tecnologias de Alto Desempenho em Ciências da Vida, Universidade Estadual de Campinas, Campinas, Brazilpt_BR
unicamp.authorArruda, P., Centro de Biologia Molecular e Engenharia Genética, Universidade Estadual de Campinas, Campinas, Brazil, Departamento de Genética e Evolução, Instituto de Biologia, Universidade Estadual de Campinas, Campinas, Brazilpt_BR
dc.description.abstractSequencing plant genomes are often challenging because of their complex architecture and high content of repetitive sequences. Sugarcane has one of the most complex genomes. It is highly polyploid, preserves intact homeologous chromosomes from its parental species and contains >55% repetitive sequences. Although bacterial artificial chromosome (BAC) libraries have emerged as an alternative for accessing the sugarcane genome, sequencing individual clones is laborious and expensive. Here, we present a strategy for sequencing and assembly reads produced from the DNA of pooled BAC clones. A set of 178 BAC clones, randomly sampled from the SP80-3280 sugarcane BAC library, was pooled and sequenced using the Illumina HiSeq2000 and PacBio platforms. A hybrid assembly strategy was used to generate 2,451 scaffolds comprising 19.2 MB of assembled genome sequence. Scaffolds of ≥20 Kb corresponded to 80% of the assembled sequences, and the full sequences of forty BACs were recovered in one or two contigs. Alignment of the BAC scaffolds with the chromosome sequences of sorghum showed a high degree of collinearity and gene order. The alignment of the BAC scaffolds to the 10 sorghum chromosomes suggests that the genome of the SP80-3280 sugarcane variety is ∼19% contracted in relation to the sorghum genome. In conclusion, our data show that sequencing pools composed of high numbers of BAC clones may help to construct a reference scaffold map of the sugarcane genome. © 2016 Okura, Souza, de Siqueira Tada and Arruda.en
dc.relation.ispartofFrontiers in Plant Sciencept_BR
dc.publisherFrontiers Research Foundationpt_BR
dc.date.issued2016pt_BR
dc.identifier.citationFrontiers In Plant Science. Frontiers Research Foundation, v. 7, p. , 2016.pt_BR
dc.language.isoenpt_BR
dc.description.volume7pt_BR
dc.description.issuenumberpt_BR
dc.description.lastpagept_BR
dc.rightsabertopt_BR
dc.sourceScopuspt_BR
dc.identifier.issn1664462Xpt_BR
dc.identifier.doi10.3389/fpls.2016.00342pt_BR
dc.identifier.urlhttps://www.scopus.com/inward/record.uri?eid=2-s2.0-84961734401&partnerID=40&md5=d981048c3162b8490a9d39a95dbfc59cpt_BR
dc.date.available2016-12-06T17:43:02Z-
dc.date.accessioned2016-12-06T17:43:02Z-
dc.description.provenanceMade available in DSpace on 2016-12-06T17:43:02Z (GMT). No. of bitstreams: 1 2-s2.0-84961734401.pdf: 359972 bytes, checksum: 4f601f7eb1707f06489c60642a37d444 (MD5) Previous issue date: 2016en
dc.identifier.urihttp://repositorio.unicamp.br/jspui/handle/REPOSIP/319252-
dc.identifier.idScopus2-s2.0-84961734401pt_BR
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