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dc.contributor.CRUESPUNIVERSIDADE DE ESTADUAL DE CAMPINASpt_BR
dc.typeresearch-articlept_BR
dc.titleHost inflammatory response to polypropylene implants: insights from a quantitative immunohistochemical and birefringence analysis in a rat subcutaneous modelpt_BR
dc.title.alternativeen
dc.contributor.authorPrudentept_BR
dc.contributor.authorAlessandro; Fávaropt_BR
dc.contributor.authorWágner José; Latuf Filhopt_BR
dc.contributor.authorPaulo; Riccettopt_BR
dc.contributor.authorCássio Luis Zanettinipt_BR
unicamp.author[Latuf Filho, Paulo] Universidade Estadual de Campinas, Brazilpt_BR
unicamp.author[Fávaro, Wágner José] Universidade Estadual de Campinas, Brazilpt_BR
unicamp.authorRiccetto, Cássio Luis Zanettini] Universidade Estadual de Campinas, Brazilpt_BR
unicamp.author.external[Prudente, Alessandropt
dc.description.abstractObjectives To describe acute and sub acute aspects of histological and immunohistochemical response to PP implant in a rat subcutaneous model based on objective methods. Materials and Methods Thirty rats had a PP mesh subcutaneously implanted and the same dissection on the other side of abdomen but without mesh (sham). The animals were euthanized after 4 and 30 days. Six slides were prepared using the tissue removed: one stained with hematoxylin-eosin (inflammation assessment); one unstained (birefringence evaluation) and four slides for immunohistochemical processing: IL-1 and TNF-? (pro-inflammatory cytokines), MMP-2 (collagen metabolism) and CD-31 (angiogenesis). The area of inflammation, the birefringence index, the area of immunoreactivity and the number of vessels were objectively measured. Results A larger area of inflammatory reaction was observed in PP compared to sham on the 4th and on the 30th day (p=0.0002). After 4 days, PP presented higher TNF (p=0.0001) immunoreactivity than sham and no differences were observed in MMP-2 (p=0.06) and IL-1 (p=0.08). After 30 days, a reduction of IL-1 (p=0.010) and TNF (p=0.016) for PP and of IL-1 (p=0.010) for sham were observed. Moreover, area of MMP-2 immunoreactivity decreased over time for PP group (p=0.018). Birefringence index and vessel counting showed no differences between PP and sham (p=0.27 and p=0.58, respectively). Conclusions The implantation of monofilament and macroporous polypropylene in the subcutaneous of rats resulted in increased inflammatory activity and higher TNF production in the early post implant phase. After 30 days, PP has similar cytokines immunoreactivity, vessel density and extracellular matrix organization.en
dc.description.abstractpt
dc.relation.ispartofInternational braz j urolpt_BR
dc.relation.ispartofInternational braz j urolpt_BR
dc.date.issued2016pt_BR
dc.identifier.citationInternational Braz J Urol, 42, 3, p.585-. 2016.pt_BR
dc.language.isoEnglishpt_BR
dc.description.volume42pt_BR
dc.description.issuenumber3pt_BR
dc.description.firstpage585pt_BR
dc.description.lastpage593pt_BR
dc.rightsabertopt_BR
dc.sourceSciELOpt_BR
dc.identifier.issn1677-6119pt_BR
dc.identifier.idScieloS1677-55382016000300585pt_BR
dc.identifier.doi10.1590/S1677-5538.IBJU.2015.0289pt_BR
dc.identifier.urlhttp://www.scielo.br/scielo.php?script=sci_arttext&pid=S1677-55382016000300585pt_BR
dc.date.available2016-11-10T19:22:51Z-
dc.date.accessioned2016-11-10T19:22:51Z-
dc.description.provenanceMade available in DSpace on 2016-11-10T19:22:51Z (GMT). No. of bitstreams: 1 S1677-55382016000300585.pdf: 7434934 bytes, checksum: 59a64e734bc173e358c5e0f468679eae (MD5) Previous issue date: 2016en
dc.identifier.urihttp://repositorio.unicamp.br/jspui/handle/REPOSIP/318972-
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