Please use this identifier to cite or link to this item: http://repositorio.unicamp.br/jspui/handle/REPOSIP/28936
Type: Artigo de periódico
Title: Quantificação de antibióticos beta-lactâmicos em medicamentos veterinários: determinação de amoxicilina e ampicilina por cromatografia líquida de alta eficiência
Title Alternative: Quantification of beta-lactam antibiotics in veterinary drugs: amoxicillin and ampicillin determination by high performance liquid chromatography
Author: Miguel, Gabriela Coelho
Orlando, Eduardo Adilson
Simionato, Ana Valéria Colnaghi
Abstract: This work focused on the development and validation of an RP-HPLC-UV method for quantification of beta-lactam antibiotics in three pharmaceutical samples. Active principles analyzed were amoxicillin and ampicillin, in 3 veterinary drugs. Mobile phase comprised 5 mmol L-1 phosphoric acid solution at pH 2.00, acetonitrile with gradient elution mode and detection wavelength at 220 nm. The method was validated according to the Brazilian National Health Surveillance regulation, where linear range and linearity, selectivity, precision, accuracy and ruggedness were evaluated. Inter day precision and accuracy for pharmaceutical samples 1, 2 and 3 were: 1.43 and 1.43%; 4.71 and 3.74%; 2.72 and 1.72%, respectively, while regression coefficients for analytical curves exceeded 0.99. The method had acceptable merit figure values, indicating reliable quantification. Analyzed samples had active principle concentrations varying from -12 to +21% compared to manufacturer label claims, rendering the medicine unsafe for administration to animals.
Subject: beta-lactam antibiotics
HPLC
method validation
Editor: Sociedade Brasileira de Química
Rights: aberto
Identifier DOI: 10.1590/S0100-40422013000800020
Address: http://dx.doi.org/10.1590/S0100-40422013000800020
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-40422013000800020
Date Issue: 1-Jan-2013
Appears in Collections:Unicamp - Artigos e Outros Documentos

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