Please use this identifier to cite or link to this item: http://repositorio.unicamp.br/jspui/handle/REPOSIP/288598
Type: DISSERTAÇÃO
Degree Level: Mestrado
Title: Analise dos efeitos da interação entre S mutans e materiais restauradores esteticos : caracteristicas do biofilme em microscopio confocal de varredura a laser e propriedades de superficie dos materiais apos 30 dias
Title Alternative: Analysis of the interaction between S. mutans and esthetic restorative materials : biofilm chracteristics by confocal laser scanning microscopy and material surface properties after 30 days
Author: Fúcio, Suzana Beatriz Portugal de
Advisor: Puppin-Rontani, Regina Maria, 1959-
Abstract: Resumo: O desenvolvimento do biofilme de Streptococcus mutans sobre materiais restauradores e a biodegradação destes substratos em função dos metabólitos bacterianos podem ser influenciados pelas propriedades e características do material. A partir de uma revisão sistemática em que se verificou a carrência de estudos a respeito dos efeitos do biofilme na superície de materiais restauradores, foi proposto investigar algumas características quantitativas e qualitativas do biofilme após 30 dias de interação com materiais restauradores, além de analisar propriedades e microestrutura da superfície dos materiais que sofreram tal interação. Para cada material testado (cerâmica - C, resina composta nanoparticulada e cimentos de inúmero de vidro modificado por resina - CIVMR e convencional - CIVC), foram confeccionados 25 discos sob condições assépticas, para distribuição em 3 grupos de estocagem: 1) 100% de umidade relativa a 37ºC (n=5); 2) meio de cultura a 37ºC (BHI + 1% sacarose) (n=5); 3) biofilme de Streptococcus mutans e meio de cultura a 37ºC (n=15). Valores de dureza do grupo 1 (valores imediatos) foram obtidos previamente à estocagem, a fim de se verificar alterações ao longo do tempo quando estocados em umidade relativa apenas. Após 30 dias de estocagem, os discos do grupo 3 foram levados para observação do biofilme corado e hidratado em microscopia de varredura confocal a laser (CLSM). As imagens obtidas auxiliaram na obtenção de valores

Abstract: Streptococcus mutans biofilm development on restorative materials and biodegradation of those materials due to bacterial acids are influenced by material properties and characteristics. Since a systematic review found a deficiency concerning studies related to effects of biofilm on the surface of restorative materials, the proposition for this investigation was to analyze some quantitative and qualitative biofilm characteristics after 30-days interaction with restorative materials. In addition, it was investigated changes on the surface properties and microstructure of materials after 30-days interaction. Twenty-five disks of each material tested (ceramic - C, nanofill composite - NC, resin-modified glass ionomer - RMGIC and conventional glass ionomer cement - CGIC) were made, at aseptic conditions, and distributed in 3 storage groups: 1) 100% relative humidity at 37ºC (n=5); 2) growth medium at 37ºC (BHI + 1% sucrose) (n=5); 3) Streptococcus mutans biofilm and growth medium at 37ºC (n=15). Vickers hardness values from group 1 were obtained previously storage, in order to observe aging by relative humidity. After 30 days storage, disks were stained, kept hydrated and observed by confocal laser scanning microscopy, whose images supported to acquire values concerning biofilm thickness, bio-volume, roughness coefficient and surface to volume ratio. Qualitative analyses related to viable / non-viable cells distribution and biofilm architecture also were realized. Subsequently, all disks were ultrasonically washed and analyzed to surface roughness, hardness and microstructure. Biofilms presented a progression more viable cells in superficial regions of the biofilm to proportionally more nonviable cells in the deeper regions of the biofilms, near the disk. Besides, cellular aggregates and fluid-filled channels were observed in biofilm developed on all materials. Concerning biofilm quantitative properties, thickness was the unique with difference statistically significant among materials. C and NC accumulated thicker biofilms than RMGIC and CGIC. There was no difference statistically significant among immediate and storage groups related to C and NC surface roughness and hardness. However, group 3 of NC showed surface biodegradation microscopically. Group 1 of RMGIC and CGIC presented higher hardness values than immediate values. Nevertheless, hardness values from RMGIC group 3 decreased compared groups 1 and 2, while surface roughness values of group 3 increased statistically. Group 3 of CGIC showed higher roughness values than other groups and no difference statistically significant among three storage groups concerning hardness values. RMGIC and CGIC micrographs also demonstrated biodegradation on the surface materials. Within this study conditions, it was concluded that there was influence of restorative materials on biofilm development and influence of biofilm on the surface properties and microstructure characteristics of materials tested, being material -dependent
Subject: Materiais dentários
Microscopia eletrônica de varredura
Language: Português
Editor: [s.n.]
Date Issue: 2007
Appears in Collections:FOP - Tese e Dissertação

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