Please use this identifier to cite or link to this item: http://repositorio.unicamp.br/jspui/handle/REPOSIP/27417
Type: Artigo de periódico
Title: The induction of differentially expressed proteins of Xylella fastidiosa with citrus extract
Title Alternative: Indução de proteínas de Xylella fastidiosa expressas diferencialmente com extrato de citros
Author: Bellato, Cláudia de M.
Garcia, Andréia K.M.
Mestrinelli, Fabiana
Tsai, Siu M.
Machado, Marcos A.
Meinhardt, Lyndel W.
Abstract: An in vitro system was developed to induce and identify Xylella fastidiosa proteins that were differentially expressed in the presence of callus-derived extracts from its host, the citrus cultivar Pêra. To optimize the induction, we first developed a single culture medium for the growth of both, host and bacteria. This medium, CPXPm7, which mimics the citrus xylem sap, showed that X. fastidiosa at 72 h post-incubation had 10(8) colony forming units mL-1, while Pêra cells had the highest fresh weight content (0.79 g). After testing various methods of co-cultivation of the bacteria and host callus grown in this single medium, the best induction procedure was to grow X. fastidiosa in a solid medium amended with an extract of Pêra callus grown in CPXPm7. Analysis, by two-dimensional electrophoresis, of the X. fastidiosa proteins (120 µg of total proteins) grown in the presence of Pêra callus extract revealed 414 differentially expressed protein spots when compared to the protein profile obtained in the absence of the extract. The system developed in this study improves the induction and analysis of differentially expressed proteins of X. fastidiosa, which may be involved in pathogenicity.
Estudos in vitro foram desenvolvidos para obter proteínas de Xylella fastidiosa expressas diferencialmente na presença de calos do hospedeiro, citros cultivar Pêra. Para otimizar a indução, desenvolveu-se um meio de cultura comum, o qual foi baseado na seiva do xilema de citros, para cultivar a bacteria e os calos de Pêra. Dados mostraram, após 72 h de cultivo neste meio, 10(8) unidades formadoras de colônias de X. fastidiosa por mL, e 0,79 g de peso seco de células de Pêra. Após testar diferentes métodos de co-cultivo da bactéria com calos de Pêra neste meio, observou-se que a melhor taxa de indução ocorreu quando X. fastidiosa foi cultivada em meio sólido enriquecido com um extrato derivado dos calos de Pêra. Análise em gel bidimensional (2DE) de X. fastidiosa (120 µg) cultivadas na presença do extrato revelou 414 proteínas expressas diferencialmente quando comparado com o perfil proteico obtido na ausência do extrato.
Subject: calos
interação Xylella-hospedeiro
2DE
patogenicidade
proteína
2DE
callus
pathogenicity
protein
Xylella-citrus interaction
Editor: Sociedade Brasileira de Microbiologia
Rights: aberto
Identifier DOI: 10.1590/S1517-83822004000200012
Address: http://dx.doi.org/10.1590/S1517-83822004000200012
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822004000200012
Date Issue: 1-Sep-2004
Appears in Collections:Artigos e Materiais de Revistas Científicas - Unicamp

Files in This Item:
File Description SizeFormat 
S1517-83822004000200012.pdf334.8 kBAdobe PDFView/Open


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.