Please use this identifier to cite or link to this item: http://repositorio.unicamp.br/jspui/handle/REPOSIP/27308
Type: Artigo de periódico
Title: Molecular matching of red blood cells is superior to serological matching in sickle cell disease patients
Author: Costa, Daiane Cobianchi da
Pellegrino Jr, Jordão
Guelsin, Gláucia Andréia Soares
Ribeiro, Karina Antero Rosa
Gilli, Simone Cristina Olenscki
Castilho, Lilian
Abstract: OBJECTIVE: To evaluate the usefulness of DNA methods to provide a means to precisely genotypically match donor blood units for the antigen-negative type of 35 sickle cell disease patients<. METHODS: Red blood cell units were investigated for ABO, D, C, c, E, e, K, Fyª, Fy b, Jkª, Jk b, S, s, Diª and RH variants by performing a molecular array (Human Erythrocyte Antigen BeadChipTM, BioArray Solutions), polymerase chain reaction followed by restriction fragment length polymorphism analysis and sequencing of patient samples and donor units that had been serologically matched based on the ABO, Rh and K phenotypes and the presence of antibodies. RESULTS: Matches for 21 of 35 sickle cell disease patients presented discrepancies or mismatches for multiple antigens between the genotype profile and the antigen profile of their serologically-matched blood units. The main discrepancies or mismatches occurred in the RH, FY, JK and MNS systems. Eight Rh alloimmunized patients presented RHD and RHCE variants that had not been serologically identified. According to these results better matches were found for the patients with genotyped units and the patients benefited as shown by better in vivo red blood cell survival. CONCLUSION: Molecular matching is superior to serological matching in sickle cell disease patients, decreasing the risk of transfusion reactions, especially delayed transfusion reactions to existing alloantibodies and preventing alloimmunization.
Subject: Anemia, sickle cell
Molecular typing
Blood group antigens
Isoantibodies
Editor: Associação Brasileira de Hematologia e Hemoterapia e daSociedade Brasileira de Transplante de Medula Óssea
Rights: aberto
Identifier DOI: 10.5581/1516-8484.20130012
Address: http://dx.doi.org/10.5581/1516-8484.20130012
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-84842013000100012
Date Issue: 1-Jan-2013
Appears in Collections:Artigos e Materiais de Revistas Científicas - Unicamp

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