Please use this identifier to cite or link to this item: http://repositorio.unicamp.br/jspui/handle/REPOSIP/27294
Type: Artigo de periódico
Title: Electrical properties of the red blood cell membrane and immunohematological investigation
Author: Fernandes, Heloise Pöckel
Cesar, Carlos Lenz
Barjas-Castro, Maria de Lourdes
Abstract: Hemagglutination is widely used in transfusion medicine and depends on several factors including antigens, antibodies, electrical properties of red blood cells and the environment of the reaction. Intermolecular forces are involved in agglutination with cell clumping occurring when the aggregation force is greater than the force of repulsion. Repulsive force is generated by negative charges on the red blood cell surface that occur due to the presence of the carboxyl group of sialic acids in the cell membrane; these charges create a repulsive electric zeta potential between cells. In transfusion services, specific solutions are used to improve hemagglutination, including enzymes that reduce the negative charge of red blood cells, LISS which improves the binding of antibodies to antigens and macromolecules that decrease the distance between erythrocytes. The specificity and sensitivity of immunohematological reactions depend directly on the appropriate use of these solutions. Knowledge of the electrical properties of red blood cells and of the action of enhancement solutions can contribute to the immunohematology practice in transfusion services.
Subject: Zeta potential
Erythrocytes
Optical tweezers
Agglutination
Editor: Associação Brasileira de Hematologia e Hemoterapia e daSociedade Brasileira de Transplante de Medula Óssea
Rights: aberto
Identifier DOI: 10.5581/1516-8484.20110080
Address: http://dx.doi.org/10.5581/1516-8484.20110080
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-84842011000400015
Date Issue: 1-Jan-2011
Appears in Collections:Artigos e Materiais de Revistas Científicas - Unicamp

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