Please use this identifier to cite or link to this item: http://repositorio.unicamp.br/jspui/handle/REPOSIP/26855
Type: Artigo de periódico
Title: Production of monoclonal antibodies for Avian Metapneumovirus (SHS-BR-121) isolated in Brazil
Author: Coswig, LT
Stach-Machado, DR
Arns, CW
Abstract: Avian Metapneumovirus (aMPV), also called Turkey Rhinotracheitis Virus (TRTV), is an upper respiratory tract infection of turkeys, chickens and other avian species. Five monoclonal antibodies (MAbs) were created against the Brazilian isolate (SHS-BR-121) of aMPV, MAbs 1A5B8; 1C1C4; 2C2E9 and 2A4C3 of IgG1 and MAb 1C1F8 of IgG2a. Four Mabs (1A5B8; 1C1C4; 2C2E9 and 2A4C3) showed neutralizing activity and three (1A5B8; 1C1C4 and 2A4C3) inhibited cellular fusion in vitro. These MAbs were used to investigate antigenic relationship among three strains (SHS-BR-121, STG 854/88 and TRT 1439/91) of aMPV subtypes A and B using cross-neutralization test. The results confirm that the monoclonal antibodies described can be used as a valuable tool in the epizootiological and serological studies, and also for the specific diagnosis of the subtypes in the infection for Avian Metapneumovirus.
Subject: Avian metapneumovirus
Brazil
monoclonal antibodies
neutralizing epitopes
Editor: Fundação APINCO de Ciência e Tecnologia Avícolas
Rights: aberto
Identifier DOI: 10.1590/S1516-635X2007000400008
Address: http://dx.doi.org/10.1590/S1516-635X2007000400008
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2007000400008
Date Issue: 1-Dec-2007
Appears in Collections:Artigos e Materiais de Revistas Científicas - Unicamp

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