Please use this identifier to cite or link to this item: http://repositorio.unicamp.br/jspui/handle/REPOSIP/26165
Type: Artigo de periódico
Title: INTRASPECIFIC VARIATION IN NEUROTOXIC AND MYOTOXIC ACTIVITIES OF Bothrops neuwiedii VENOMS
Author: BORJA-OLIVEIRA, C. R.
SOARES, A. M.
ZAMUNÉR, S. R.
HYSLOP, S.
GIGLIO, J. R.
PRADO-FRANCESCHI, J.
RODRIGUES-SIMIONI, L.
Abstract: Snake venoms frequently vary in composition. In this work, we compared the neurotoxic and myotoxic activities of 16 lots of Bothrops neuwiedii venoms from different regions of Brazil, using chick biventer cervicis preparations. The neuromuscular blockade varied from 2% to 100% after 120 min incubation with venoms (50 mug/ml). In all cases, this blockade was irreversible and concentration-dependent; at low concentrations (10-20 mug/ml), 15 of the 16 venom lots failed to abolish responses to acetylcholine (110 muM), but blocked responses to KCl (13.4 muM), and induced contracture. At 5-20 mug/ml, the most active venom totally blocked twitch-tension without affecting responses to acetylcholine and KCl. Polyacrylamide gel electrophoresis for basic proteins showed that the most active samples contained a band that was absent in the less active venoms. These results indicate that there may be considerable intraspecific variation in the neurotoxic activity of B. neuwiedii venoms, whereas myotoxic activity is less variable.
Subject: Bothrops neuwiedii venom
intraspecific variation
myotoxicity
neurotoxicity
neuromuscular blockade
Editor: Centro de Estudos de Venenos e Animais Peçonhentos - CEVAP, Universidade Estadual Paulista - UNESP
Rights: aberto
Identifier DOI: 10.1590/S0104-79302002000100008
Address: http://dx.doi.org/10.1590/S0104-79302002000100008
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0104-79302002000100008
Date Issue: 1-Jan-2002
Appears in Collections:Artigos e Materiais de Revistas Científicas - Unicamp

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