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Type: Artigo de periódico
Title: The effect of medium composition on interleukin-2 production by murine EL-4 thymoma cells
Author: Galesi, A. L. L.
Tamashiro, W. M. S. C.
Moraes, A. M.
Abstract: Due to the role of interleukin-2 (IL-2) in the mediation of immune response, this cytokine has been used in the treatment of some types of cancer and infectious diseases. However, relatively high levels of this cytokine are required to achieve significant activity. The aim of this work was to study a culture medium composition designed to increase the production of IL-2 by suspended murine EL-4 cells. The cultivations were carried out aiming at producing IL-2 in stirred bioreactors. The effects of concentration of glutamine, phorbol-12-myristate-13-acetate (PMA), concanavalin A (Con A), Pluronic F68, and fetal calf serum (FCS) on cell viability and IL-2 production were evaluated. PMA alone was more efficient in IL-2 production than it was in association with Con A. The maximum IL-2 production was around 162 ng/mL with 856 ng/mL PMA and 1.45% (v/v) FCS.
Subject: cytokine
Editor: Brazilian Society of Chemical Engineering
Rights: aberto
Identifier DOI: 10.1590/S0104-66322004000200005
Date Issue: 1-Jun-2004
Appears in Collections:Artigos e Materiais de Revistas Científicas - Unicamp

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