Please use this identifier to cite or link to this item: http://repositorio.unicamp.br/jspui/handle/REPOSIP/26064
Type: Artigo de periódico
Title: Aprotinin recovery: comparison between biospecific and pseudobiospecific affinity adsorptions
Author: TAMAGAWA, R. E.
AZZONI, A. R.
MIRANDA, E. A.
VIJAYALAKSHMI, M. A.
Abstract: Two adsorption techniques were studied as potential methods for the recovery of aprotinin: a biospecific adsorption (using immobilized trypsin and chymotrypsin as ligants) and a pseudobiospecific adsorption of aprotinin-trypsin complex onto IMAC matrix. These studies indicated that ionic strength has an important effect on aprotinin adsorption on immobilized trypsin, and the pH was the most significant variable in aprotinin desorption from both immobilized enzymes. The aprotinin-trypsin complex adsorption onto the IMAC matrix was not as significantly affected by changes in pH as it was for changes in ionic strength. For the desorption step, both variables significantly affected the complex recovery. However, the effect of ionic strength was markedly stronger for this desorption step.
Subject: Aprotinin recovery
adsorptions
IMAC matrix
Editor: Brazilian Society of Chemical Engineering
Rights: aberto
Identifier DOI: 10.1590/S0104-66321999000200003
Address: http://dx.doi.org/10.1590/S0104-66321999000200003
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0104-66321999000200003
Date Issue: 1-Jun-1999
Appears in Collections:Unicamp - Artigos e Outros Documentos

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