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Type: Artigo de periódico
Title: Production of lipase from Geotrichum sp and adsorption studies on affinity resin
Author: KAMIMURA, E. S.
Abstract: There is a growing interest in microbial lipase production due to its great potential for industrial applications such as food additives, industrial reagents and stain removers, as well as for medical applications. Specially for medical applications a high degree of purity is required, which is accomplished with high resolution chromatographic techniques. Affinity chromatography is considered a very high resolution chromatographic technique. In this work the adsorption isotherms and kinetics of the adsorption of lipase from Geotrichum sp on biospecific resin were determined. The resin was prepared using EAH sepharose 4B gel (Pharmacia), made to react with oleic acid as the specific ligand.The lipase was produced in a five-liter fermenter, with both complex and synthetic media. Fermentation conditions were a temperature of 30°C, an aeration of 1VVM and an agitation of 400 rpm. Maximum lipase activity was around 28 U/ml after 10 hours of fermentation for the complex medium. The kinetic model and parameters were determined by dynamic fitting to experimental results using the fourth-order Runge-Kutta method.
Subject: lipase
affinity resin
kinetic constant
Editor: Brazilian Society of Chemical Engineering
Rights: aberto
Identifier DOI: 10.1590/S0104-66321999000200001
Date Issue: 1-Jun-1999
Appears in Collections:Artigos e Materiais de Revistas Científicas - Unicamp

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