Please use this identifier to cite or link to this item: http://repositorio.unicamp.br/jspui/handle/REPOSIP/25865
Type: Artigo de periódico
Title: Associação entre HLA e leucemia em uma população brasileira de etnia mista
Title Alternative: Association between HLA and leukemia in a mixed Brazilian population
Author: Barion, Lúcia Aparecida
Tsuneto, Luiza Tamie
Testa, Giuliana Vitoriano
Lieber, Sofia Rocha
Persoli, Ligia Beatriz Lopes
Marques, Silvia Barbosa Dutra
Vigorito, Afonso Celso
Aranha, Francisco José Penteado
Eid, Katia Aparecida Brito
Oliveira, Gislaine Borba
Miranda, Eliana Cristina Martins
Souza, Cármino Antonio de
Visentainer, Jeane Eliete Laguila
Abstract: OBJECTIVE: The main purpose of this study was to investigate the class I HLA antigens and class II HLA allele frequencies in 164 patients with leukemia: 35 patients with ALL (acute lymphoid leukemia), 50 with AML (acute myeloid leukemia) and 78 with CML (chronic myeloid leukemia). METHODS: The genotyping of class I HLA was performed by microlymphocytotoxicity and of class II by PCR-SSP (polymerase chain reaction - sequence specific of primers) (One Lambda, Canoga Park, CA, USA). RESULTS: In patients with LLA, frequencies of HLA-B45 and HLA-B56 were higher (P = 0.02; OR = 3.13; 95%IC = 0.94-10.44; P = 0.03; OR = 3.61; 95%IC = 0.47-27.64, respectively), than in controls. In patients with AML, the frequency of HLA-B7 (P = 0.01; OR = 2.41; 95%IC = 1.25-4.67) was higher than in controls. The presence of HLA-B45 (P= 0.01; OR = 3.29; 95%IC = 1.46-7.40), HLA-DRB1*04 (P = 0.002; OR = 2.17; 95%IC = 1.36-3.46) and HLA-DRB1*08 (P = 0.004; OR = 2.36; 95%IC = 1.34-4.16) was associated to increased risk of CML developing. CONCLUSION: Our results suggest that variants of HLA confer susceptibility to the same forms of leukemia, and could provide new tools for the investigation of genetics and etiology of this disease.
OBJETIVOS: O objetivo deste estudo foi investigar a freqüência de antígenos HLA Classe I e de alelos HLA Classe II em 164 pacientes com vários tipos de leucemias: 35 pacientes com LLA (leucemia linfóide aguda), 50 com LMA (leucemia mielóide aguda) e 78 com LMC (leucemia mielóide crônica). MÉTODOS: A tipagem HLA Classe I foi realizada por microlinfocitotoxicidade e a de Classe II por PCR-SSP (polymerase chain reaction - sequence specific of primers), ambas da One Lambda (Canoga Park, CA, US). RESULTADOS: Em pacientes com LLA, as freqüências das variantes HLA-B45 e HLA-B56 foram maiores (P = 0,02; OR = 3,13; 95%IC = 0,94-10,44; P = 0,03; OR = 3,61; 95%IC = 0,47-27,64, respectivamente), quando comparadas com controles. Nos pacientes com LMA, a freqüência de HLA-B7 (P = 0,01; OR = 2,41; 95%IC = 1,25-4,67) foi maior que em controles. A presença de HLA-B45 (P= 0,01; OR = 3,29; 95%IC = 1,46-7,40) e de HLA-DRB1*04 (P = 0,002; OR = 2,17; 95%IC = 1,36-3,46) e HLA-DRB1*08 (P = 0,004; OR = 2,36; 95%IC = 1,34-4,16) foi associada ao maior risco de desenvolver LMC. CONCLUSÃO: Nossos resultados sugerem que variantes HLA conferem susceptibilidade a algumas formas de leucemia e podem prover novas ferramentas para a investigação da genética e etiologia desta doença.
Subject: HLA
Leucemia
Associação genética
HLA
Leukemia
Genetic association
Editor: Associação Médica Brasileira
Rights: aberto
Identifier DOI: 10.1590/S0104-42302007000300024
Address: http://dx.doi.org/10.1590/S0104-42302007000300024
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0104-42302007000300024
Date Issue: 1-Jun-2007
Appears in Collections:Artigos e Materiais de Revistas Científicas - Unicamp

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