Please use this identifier to cite or link to this item: http://repositorio.unicamp.br/jspui/handle/REPOSIP/256293
Type: TESE
Title: Avaliação dos níveis de ingestão diária de edulcorantes pelo consumo de adoçantes líquidos de mesa
Title Alternative: Evaluation of the intake of sweeteners by consuming tabletop weeteners
Author: Del Bianchi, Michelle, 1982-
Advisor: Reyes, Felix Guillermo Reyes, 1948-
Abstract: Resumo: Edulcorantes sao compostos quimicos de origem sintetica ou natural que tem a propriedade de adocar alimentos, em substituicao total ou parcial a sacarose ou outros acucares e reduzem o teor calorico do produto resultante. A ingestao de adocantes artificiais no mundo tem crescido nos ultimos anos, com o objetivo principal de reduzir a ingestao calorica. O aumento global de doencas cronicas relacionadas com a ingestao de acucar, particularmente a obesidade e a diabetes, e tambem uma razao para o aumento do consumo de adocantes artificiais. O objetivo deste estudo foi avaliar a ingestao de adocantes artificiais atraves do consumo de adocantes de mesa liquidos e avaliar a possibilidade de consumo superior a sua ingestao diaria aceitavel (IDA) estabelecida por FAO/WHO comite de peritos em aditivos alimentares (JECFA). Inicialmente, uma pesquisa de campo foi realizada para avaliar qual marca de adocante de mesa liquido mais consumido no Brasil e as caracteristicas de uso do mesmo. Para tanto, um questionario foi enviado por correio eletronico, para individuos, com questoes relativas ao consumo de adocantes e, caso consumidos, aspectos de uso incluindo marca do adocante, ingestao e motivo de consumo. O questionario tambem continha perguntas sobre questoes socioeconomicas, demograficas e de saude. Em base a esta pesquisa, verificou-se quais sao as marcas de adocantes liquidos de mesa mais consumidas. Os edulcorantes presentes nesses adocantes mais consumidos sao: sacarina, ciclamato, aspartame e acessulfame-K. Foi realizada a quantificacao desses edulcorantes nos adocantes de maior consumo. Para a quantificacao de sacarina, aspartame e acessulfame-K foi utilizada a cromatografia liquida de alta eficiencia acoplada ao detector de arranjo de diodos (CLAE-DAD). A quantificacao de ciclamato foi realizada por espectrofotometria UV/Vis, em 314 nm, apos derivatizacao para N, Ndiclorociclohexilamina. Os metodos analiticos foram validados de acordo com os seguintes parametros de validacao: linearidade, faixa linear, especificidade, precisao (repetibilidade intra e inter dias), limite de deteccao (LOD) e limite de quantificacao (LOQ). Todos os metodos mostraram parametros de validacao de acordo com o guia para validacao de metodos analiticos estabelecidos pela Agencia Nacional de Vigilancia Sanitaria (ANVISA). A maioria das respostas ao questionario foram do genero feminio (70,2%) e provenientes da regiao Sudeste do Brasil (70,8%). Entre os entrevistados, 47% usavam adocantes liquidos de mesa. A maioria que relatou o uso de adocantes artificiais tinham entre 21 e 32 anos ou acima de 45 anos de idade. Destes, 83% afirmaram nao ter qualquer tipo de patologia e que a principal razao para o consumo de adocantes liquidos de mesa foi a preferencia em relacao ao acucar. Cerca da metade (54%) dos consumidores relataram sentir sabor desagradavel ou amargo quando consomem adocante e 32% nao sabiam qual era o edulcorante artificial contido no adocante. Os resultados da analise dos adocantes liquidos de mesa provenientes do mercado varejista indicou variacao significativa (p<0,05) na concentracao de edulcorante entre marcas e lotes da mesma marca. Com base nos resultados do questionario verificou-se qual e a marca mais consumida. Essa marca contem sacarina e ciclamato. Uma amostra dessa marca foi entregue a individuos adultos consumidores deste produto, para calcular o nivel de consumo dos edulcorantes artificiais. Foi verificado que a ingestao de sacarina e ciclamato atraves do consumo de adocantes liquidos de mesa representa, em media, 38,7% e 19,8% do valor de IDA do edulcorante, respectivamente. Considerando a variacao na concentracao de adocantes entre as marcas e lotes da mesma marca e que cada edulcorante artificial tem um valor de IDA estabelecido, recomenda-se que as concentracoes dos edulcorante em adocantes liquidos de mesa sejam apresentados na etiqueta da embalagem, para fornecer ao consumidor informacoes sobre seu consumo, e permitir controlar a sua exposicao total a estas substancias. Em geral, os resultados sugerem uma substituicao cada vez maior de acucar pelos adocantes artificiais, que indicam a necessidade de se obter dados sobre a ingestao total de adocantes artificiais, a fim de avaliar o risco de que estas substancias apresentam para a saude dos consumidores

Abstract: Artificial sweeteners are chemical compounds of synthetic or natural origin that have sweetening properties, as full or partial replacement of sucrose or other sugars, and reduce the caloric content of the resulting product. The intake of artificial sweeteners in the world has grown in recent years, with the primary goal of reducing caloric intake. The overall increase of chronic diseases relating to sugar intake, particularly obesity and diabetes, is also a reason for the increased consumption of artificial sweeteners. The aim of this study was to evaluate the intake of artificial sweeteners through the consumption of liquid tabletop sweeteners and evaluate the possibility of consumption exceeding their Acceptable Daily Intake (ADI) established by the FAO/WHO Joint Expert Committee on Food Additives (JECFA). Initially, a field survey was undertaken to assess which liquid tabletop sweetener brand had the largest market share of the retail market in Brazil and the characteristics of usage. For this purpose, a questionnaire was submitted by electronic mail to individuals with questions relating to their consumption of artificial sweeteners, and if consumed, aspects of usage including brand, frequency and motivation of consumption. In addition, the questionnaire contained questions regarding socio-economic, demographic and health status. Based on the results of the survey the specific artificial sweeteners present in the most widely used liquid tabletop sweetener brands were identified and quantified. The artificial sweeteners present in the most widely consumed brands are: saccharin, cyclamate, aspartame and acesulfame-K. Quantification of saccharin, aspartame and acesulfame-K in the commercial products was determined by high performance liquid chromatography coupled to a diode array detector (HPLC-DAD). Quantification of cyclamate was carried out by spectrophotometry UV/Vis, at 314 nm, after derivatization to N, N diclorocyclohexilamine. The analytical methods employed were validated according to the following validation parameters: linearity, linear range, specificity, accuracy, precision (repeatability intra-and inter-day), limit of detection (LOD) and limit of quantification (LOQ). All the methods showed validation parameters in accordance to the Guide for the Validation of Analytical Methods established by the Brazilian Agency of Sanitary Surveillance (ANVISA). Most of the responses to the questionnaire came from females (70.2%), from the southeast region of Brazil (70.8%). Among the respondents, 47% used artificial tabletop sweeteners. The majority of the respondents who reported using artificial sweeteners were between 21 and 32 years old or above 45 years old. Among these 83% reported not having any type of pathology and that the primary reason for artificial sweetener consumption was the preference in relation to sugar. Approximately half (54%) of consumers reported an unpleasant or bitter aftertaste when consuming the sweetener and 32% did not know which artificial sweetener was in the sweetener they used. The results of the analysis of the liquid tabletop sweeteners samples from the retail market indicated there is a statistical variation (p<0.05) in the sweeteners concentration between brands and batches from the same brand. Based on the questionnaire results, it was verified the most consumed brand. The brand contains saccharin and cyclamate. A sample of this brand was provided to adult volunteers to estimate the level of consumption of those artificial sweeteners. It was found that the intake of saccharin and cyclamate through the consumption of the liquid tabletop sweetener represent, on average, 38.7% and 19.8% of their ADI values, respectively. Considering the variation found in the sweeteners concentration between brands and batches from the same brand and that each artificial sweetener has an ADI value established, it is recommended that the concentrations of the artificial sweeteners in the liquid tabletop sweeteners be shown on the packaging label, to allow the consumer to make informed decisions regarding their intake and to allow them to better control their total exposure to these substances. In general, the results suggest an increasing replacement of sugar by artificial sweeteners, which indicate the need to obtain up to date data on the total intake of artificial sweeteners in order to assess the risk that these substances present to the consumers health
Subject: Adoçantes
Pesquisas de campo
Cromatografia líquida de alta eficiência
Espectrofotometria
Language: Português
Editor: [s.n.]
Date Issue: 2012
Appears in Collections:FEA - Dissertação e Tese

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