Please use this identifier to cite or link to this item: http://repositorio.unicamp.br/jspui/handle/REPOSIP/25622
Type: Artigo de periódico
Title: Genetic diversity analysis among pigeonpea genotypes adapted to South American regions based on microsatellite markers
Author: Sousa, Adna Cristina Barbosa de
Godoy, Rodolfo
Sforça, Danilo Augusto
Campos, Tatiana de
Zucchi, Maria Imaculada
Jank, Liana
Souza, Anete Pereira de
Abstract: The pigeonpea [Cajanus cajan (L) Millspaugh] is one of the most important perennial legume crops utilized in the food, fodder, soil conservation, crop-livestock integrated systems, reclaiming of degraded pastures and symbiotic nitrogen fixation. Microsatellite markers were used to estimate the genetic diversity of 77 pigeonpea genotypes selected from the germplasm collections at Embrapa Cattle-Southeast and, to evaluate their transferability to Phaseolus vulgaris and Vigna unguiculata species. The number of alleles per locus ranged from 2 to12, with an average of 5.1 alleles. The PIC values ranged from 0.11 to 0.80 (average 0.49) and the D values from 0.23 to 0.91 (average 0.58). The averages of observed and expected heterozygosity were 0.25 and 0.47, respectively, showing a deficit in heterozygosity. A model-based Bayesian approach implemented in the software STRUCTURE was used to assign genotypes into clusters. A dendrogram was constructed based on the modified Roger's genetic distances using a neighbor-joining method (NJ). A total of four clusters were assembled by STRUCTURE and a strong tendency of correspondence between the Bayesian clusters in the NJ tree was observed. The genetic distance ranged from 0.09 to 0.62 (average 0.37), showing a low genetic diversity in the pigeonpea genotypes. Transferability of pigeonpea-specific microsatellites revealed a cross-amplification and the presence of polymorphic alleles in P. vulgaris and V. unguiculata.
Subject: legumes
transferability
microsatellite
germplasm
Editor: São Paulo - Escola Superior de Agricultura "Luiz de Queiroz"
Rights: aberto
Identifier DOI: 10.1590/S0103-90162011000400007
Address: http://dx.doi.org/10.1590/S0103-90162011000400007
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162011000400007
Date Issue: 1-Aug-2011
Appears in Collections:Artigos e Materiais de Revistas Científicas - Unicamp

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