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Type: Artigo de periódico
Title: Vapd In Xylella Fastidiosa Is A Thermostable Protein With Ribonuclease Activity
Author: Mendes
Juliano S.; Santiago
Andre da S.; Toledo
Marcelo A. S.; Rosselli-Murai
Luciana K.; Favaro
Marianna T. P.; Santos
Clelton A.; Horta
Maria Augusta C.; Crucello
Aline; Beloti
Lilian L.; Romero
Fabian; Tasic
Ljubica; de Souza
Alessandra A.; de Souza
Anete P.
Abstract: Xylella fastidiosa strain 9a5c is a gram-negative phytopathogen that is the causal agent of citrus variegated chlorosis (CVC), a disease that is responsible for economic losses in Brazilian agriculture. The most well-known mechanism of pathogenicity for this bacterial pathogen is xylem vessel occlusion, which results from bacterial movement and the formation of biofilms. The molecular mechanisms underlying the virulence caused by biofilm formation are unknown. Here, we provide evidence showing that virulence-associated protein D in X. fastidiosa (Xf-VapD) is a thermostable protein with ribonuclease activity. Moreover, protein expression analyses in two X. fastidiosa strains, including virulent (Xf9a5c) and nonpathogenic (XfJ1a12) strains, showed that Xf-VapD was expressed during all phases of development in both strains and that increased expression was observed in Xf9a5c during biofilm growth. This study is an important step toward characterizing and improving our understanding of the biological significance of Xf-VapD and its potential functions in the CVC pathosystem.
Subject: Biofilm Formation
Structure Prediction
Bacterial Virulence
Antisense Rnas
Citation: Vapd In Xylella Fastidiosa Is A Thermostable Protein With Ribonuclease Activity. Public Library Science, v. 10, p. DEC-2015.
Rights: aberto
Identifier DOI: 10.1371/journal.pone.0145765
Date Issue: 2015
Appears in Collections:Unicamp - Artigos e Outros Documentos

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