Please use this identifier to cite or link to this item: http://repositorio.unicamp.br/jspui/handle/REPOSIP/244100
Type: Artigo de periódico
Title: Microbiomes Of Endodontic-periodontal Lesions Before And After Chemomechanical Preparation
Author: Gomes
Brenda P. F. A.; Berber
Vanessa B.; Kokaras
Alexis S.; Chen
Tsute; Paster
Bruce J.
Abstract: Introduction: This study was conducted to evaluate the microbiomes of endodontic-periodontal lesions before and after chemomechanical preparation (CMP). Methods: Clinical samples were taken from 15 root canals (RCs) with necrotic pulp tissues and from their associated periodontal pockets (PPs) (n = 15) of teeth with endodontic-periodontal lesions before and after CMP. The Human Oral Microbe Identification using Next Generation Sequencing (NGS) protocol and viable culture were used to analyze samples from RCs and PPs. The Mann-Whitney U test and Benjamini-Hochberg corrections were performed to correlate the clinical and radiographic findings with microbial findings (P < .05). Results: Bacteria were detected in 100% of the samples in both sites (15/15) using NGS. Firmicutes was the most predominant phylum in both sites using both methods. The most frequently detected species in the RCs before and after CMP using NGS were Enterococcus faecalis, Parvimonas micra, Mogibacterium timidum, Filifactor alocis, and Fretibacterium fastidiosum. The species most frequently detected in the PPs before and after CMP using NGS were P. micra, E. faecalis, Streptococcus constellatus, Eubacterium brachy, Tannerella forsythia, and F. alocis. Associations were found between periapical lesions <= 2 mm and Desulfobulbus sp oral taxon 041 and with periodontal pockets >= 6mm and Dialister invisius and Peptostreptococcus stomatis (all P < .05, found in the RCs before CMP). Conclusions: It is concluded that the microbial community present in combined endodontic-periodontal lesions is complex and more diverse than previously reported. It is important to note that bacteria do survive in some root canals after CMP. Finally, the similarity between the microbiota of both sites, before and after CMP, suggests there may be a pathway of infection between the pulp and periodontium.
Subject: Gradient Gel-electrophoresis
Polymerase-chain-reaction
Infected Root Canals
Enterococcus-faecalis
Teeth
Bacteria
Endotoxins
Prevalence
Pathogens
Culture
Country: NEW YORK
Editor: ELSEVIER SCIENCE INC
Citation: Microbiomes Of Endodontic-periodontal Lesions Before And After Chemomechanical Preparation. Elsevier Science Inc, v. 41, p. 1975-1984 DEC-2015.
Rights: embargo
Identifier DOI: 10.1016/j.joen.2015.08.022
Address: http://www.sciencedirect.com/science/article/pii/S0099239915007876
Date Issue: 2015
Appears in Collections:Unicamp - Artigos e Outros Documentos

Files in This Item:
File SizeFormat 
wos_000366146200005.pdf640.62 kBAdobe PDFView/Open


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.