Please use this identifier to cite or link to this item: http://repositorio.unicamp.br/jspui/handle/REPOSIP/244062
Type: Artigo de periódico
Title: Lipidomics Analysis Of Follicular Fluid By Esi-ms Reveals Potential Biomarkers For Ovarian Endometriosis
Author: Cordeiro
Fernanda Bertuccez; Cataldi
Thais Regiani; Perkel
Kayla Jane; Teixeira da Costa
Lvia do Vale; Rochetti
Raquel Cellin; Stevanato
Juliana; Eberlin
Marcos Nogueira; Zylbersztejn
Daniel Suslik; Cedenho
Agnaldo Pereira; Lo Turco
Edson Guimaraes
Abstract: The aim of the present study was to analyze the lipid profile of follicular fluid from patients with endometriosis and endometrioma who underwent in vitro fertilization treatment (IVF). The control group (n = 10) was composed of women with tubal factor or minimal male factor infertility who had positive pregnancy outcomes after IVF. The endometriosis group consisted of women with endometriosis diagnosed by videolaparoscopy (n = 10), and from the same patients, the endometriomas fluids were collected, which composed the endometrioma group (n = 10). From the follicular fluid and endometriomas, lipids were extracted by the Bligh and Dyer method, and the samples were analyzed by tandem mass spectrometry. We observed phosphatidylglycerol phosphate, phosphatidylcholine, phosphatidylserine, and phosphatidylnositol bisphosphate in the control group. In the endometriosis group, sphingolipids and phosphatidylcholines were more abundant, while in the endometrioma group, sphingolipids and phosphatidylcholines with different m/z from the endometriosis group were found in high abundance. This analysis demonstrated that there is a differential representation of these lipids according to their respective groups. In addition, the lipids found are involved in important mechanisms related to endometriosis progress in the ovary. Thus, the metabolomic approach for the study of lipids may be helpful in potential biomarker discovery.
Subject: Primate Corpus-luteum
Apoptosis
Cells
Phosphatidylserine
Fertilization
Mitochondria
Gonadotropin
Lymphocytes
Metabolism
Mechanisms
Country: NEW YORK
Editor: SPRINGER/PLENUM PUBLISHERS
Rights: fechado
Identifier DOI: 10.1007/s10815-015-0592-1
Address: http://link.springer.com/article/10.1007%2Fs10815-015-0592-1
Date Issue: 2015
Appears in Collections:Unicamp - Artigos e Outros Documentos

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