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Type: Artigo de periódico
Title: Large-scale Transcriptome Analysis Of Two Sugarcane Genotypes Contrasting For Lignin Content
Author: Vicentini
Renato; Bottcher
Alexandra; Brito
Michael dos Santos; dos Santos
Adriana Brombini; Creste
Silvana; de Andrade Landell
Marcos Guimaraes; Cesarino
Igor; Mazzafera
Abstract: Sugarcane is an important crop worldwide for sugar and first generation ethanol production. Recently, the residue of sugarcane mills, named bagasse, has been considered a promising lignocellulosic biomass to produce the second-generation ethanol. Lignin is a major factor limiting the use of bagasse and other plant lignocellulosic materials to produce second-generation ethanol. Lignin biosynthesis pathway is a complex network and changes in the expression of genes of this pathway have in general led to diverse and undesirable impacts on plant structure and physiology. Despite its economic importance, sugarcane genome was still not sequenced. In this study a high-throughput transcriptome evaluation of two sugarcane genotypes contrasting for lignin content was carried out. We generated a set of 85,151 transcripts of sugarcane using RNA-seq and de novo assembling. More than 2,000 transcripts showed differential expression between the genotypes, including several genes involved in the lignin biosynthetic pathway. This information can give valuable knowledge on the lignin biosynthesis and its interactions with other metabolic pathways in the complex sugarcane genome.
Subject: Class-iii Peroxidases
Cell-wall Composition
Coenzyme-a Ligases
Biofuel Production
Biosynthesis Perturbations
Expression Analysis
Metabolic Flux
Citation: Large-scale Transcriptome Analysis Of Two Sugarcane Genotypes Contrasting For Lignin Content. Public Library Science, v. 10, p. AUG-2015.
Rights: aberto
Identifier DOI: 10.1371/journal.pone.0134909
Date Issue: 2015
Appears in Collections:Unicamp - Artigos e Outros Documentos

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