Please use this identifier to cite or link to this item: http://repositorio.unicamp.br/jspui/handle/REPOSIP/243990
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dc.contributor.CRUESPUNIVERSIDADE DE ESTADUAL DE CAMPINASpt_BR
dc.typeArtigo de periódicopt_BR
dc.titleSpr Analysis Of The Interaction Between A Recombinant Protein Of Unknown Function In Leishmania Infantum Immobilised On Dendrimers And Antibodies Of The Visceral Leishmaniasis: A Potential Use In Immunodiagnosispt_BR
dc.contributor.authorSoutopt_BR
dc.contributor.authorDenio E. P.; Fonsecapt_BR
dc.contributor.authorAliani M.; Barraganpt_BR
dc.contributor.authorJose T. C.; Luzpt_BR
dc.contributor.authorRita de C. S.; Andradept_BR
dc.contributor.authorHelida M.; Damospt_BR
dc.contributor.authorFlavio S.; Kubotapt_BR
dc.contributor.authorLauro T.pt_BR
unicamp.author.emailKubota@iqm.unicamp.brpt_BR
unicamp.authorKubota, Lauro T.] State Univ Campinas Unicamp, Inst Chem, Natl Inst Sci & Technol Bioanalyt, Sao Paulo, SP, Brazilpt_BR
unicamp.author.external[Souto, Denio E. P.pt
unicamp.author.externalBarragan, Jose T. C.pt
unicamp.author.external[Fonseca, Aliani M.pt
unicamp.author.externalAndrade, Helida M.] Univ Fed Minas Gerais, Belo Horizonte, MG, Brazilpt
unicamp.author.external[Luz, Rita de C. S.pt
unicamp.author.externalDamos, Flavio S.] Fed Univ Maranhao UFMA, Sao Luis, MA, Brazilpt
dc.subjectTrypanosoma-cruzipt_BR
dc.subjectInfectionpt_BR
dc.subjectHostpt_BR
dc.subjectDiseasept_BR
dc.subjectSurfacept_BR
dc.description.abstractIn this work, an SPR immunosensor was developed to elucidate the reaction kinetics between a protein of unknown function in Leishmania infantum (hypothetical C1 protein) and specific antibodies of the visceral leishmaniasis (VL). A platform, which is based on layer-by-layer assembly was formed by cysteamine in combination with a fourth-generation poly(amidoamine) dendrimer (PAMAM(G4)) on gold surface for the immobilisation of the protein. This film resulted in amplification of the signal of SPR. Then, a kinetic model based on a bivalent ligation suggested that the reaction between the C1 protein and the anti-C1 antibody occurs in two steps. The value of the equilibrium dissociation constant (K-D1 x K-D2=1.64 x 10(-7) mol L-1) demonstrated high binding affinity between the biomolecules. Furthermore, low limits of detection (LOD =7.37 nmol L-1) and quantification (LOQ= 7.83 nmol L-1) were presented with the proposed SPR immunosensor. Afterwards, the addition of real samples consisting of positive and negative canine sera for VL was accompanied by high sensitivity and selectivity by SPR immunosensor. Therefore, this study quantitatively demonstrated the strong antigenic character of a hypothetical protein and consequently its potential use in the immunodiagnosis of the VL. (C) 2015 Elsevier B.V. All rights reserved.en
dc.relation.ispartofBIOSENSORS & BIOELECTRONICSpt_BR
dc.publisher.countryOXFORDpt_BR
dc.publisherELSEVIER ADVANCED TECHNOLOGYpt_BR
dc.date.issued2015-AUGpt_BR
dc.identifier.citationSpr Analysis Of The Interaction Between A Recombinant Protein Of Unknown Function In Leishmania Infantum Immobilised On Dendrimers And Antibodies Of The Visceral Leishmaniasis: A Potential Use In Immunodiagnosis. Elsevier Advanced Technology, v. 70, p. 275-281 AUG-2015.pt_BR
dc.language.isoenpt_BR
dc.description.volume70pt_BR
dc.description.firstpage275pt_BR
dc.description.lastpage281pt_BR
dc.rightsembargopt_BR
dc.sourceWOSpt_BR
dc.identifier.issn0956-5663pt_BR
dc.identifier.wosidWOS:000356554400038pt_BR
dc.identifier.doi10.1016/j.bios.2015.03.034pt_BR
dc.identifier.urlhttp://www.sciencedirect.com/science/article/pii/S0956566315001864pt_BR
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)pt_BR
dc.description.sponsorshipNational Institute of Science & Technology of Bioanalytics (INCTBio)pt_BR
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)pt_BR
dc.description.sponsorship1Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)pt_BR
dc.description.sponsorship1Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)pt_BR
dc.description.sponsordocumentnumberFAPESP [2012/24493-3]pt
dc.date.available2016-06-07T13:34:49Z-
dc.date.accessioned2016-06-07T13:34:49Z-
dc.description.provenanceMade available in DSpace on 2016-06-07T13:34:49Z (GMT). No. of bitstreams: 1 wos_000356554400038.pdf: 832472 bytes, checksum: 32d7f2f835da80a07abe00609f1feae1 (MD5) Previous issue date: 2015en
dc.identifier.urihttp://repositorio.unicamp.br/jspui/handle/REPOSIP/243990-
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