Please use this identifier to cite or link to this item: http://repositorio.unicamp.br/jspui/handle/REPOSIP/243990
Type: Artigo de periódico
Title: Spr Analysis Of The Interaction Between A Recombinant Protein Of Unknown Function In Leishmania Infantum Immobilised On Dendrimers And Antibodies Of The Visceral Leishmaniasis: A Potential Use In Immunodiagnosis
Author: Souto
Denio E. P.; Fonseca
Aliani M.; Barragan
Jose T. C.; Luz
Rita de C. S.; Andrade
Helida M.; Damos
Flavio S.; Kubota
Lauro T.
Abstract: In this work, an SPR immunosensor was developed to elucidate the reaction kinetics between a protein of unknown function in Leishmania infantum (hypothetical C1 protein) and specific antibodies of the visceral leishmaniasis (VL). A platform, which is based on layer-by-layer assembly was formed by cysteamine in combination with a fourth-generation poly(amidoamine) dendrimer (PAMAM(G4)) on gold surface for the immobilisation of the protein. This film resulted in amplification of the signal of SPR. Then, a kinetic model based on a bivalent ligation suggested that the reaction between the C1 protein and the anti-C1 antibody occurs in two steps. The value of the equilibrium dissociation constant (K-D1 x K-D2=1.64 x 10(-7) mol L-1) demonstrated high binding affinity between the biomolecules. Furthermore, low limits of detection (LOD =7.37 nmol L-1) and quantification (LOQ= 7.83 nmol L-1) were presented with the proposed SPR immunosensor. Afterwards, the addition of real samples consisting of positive and negative canine sera for VL was accompanied by high sensitivity and selectivity by SPR immunosensor. Therefore, this study quantitatively demonstrated the strong antigenic character of a hypothetical protein and consequently its potential use in the immunodiagnosis of the VL. (C) 2015 Elsevier B.V. All rights reserved.
Subject: Trypanosoma-cruzi
Infection
Host
Disease
Surface
Country: OXFORD
Editor: ELSEVIER ADVANCED TECHNOLOGY
Citation: Spr Analysis Of The Interaction Between A Recombinant Protein Of Unknown Function In Leishmania Infantum Immobilised On Dendrimers And Antibodies Of The Visceral Leishmaniasis: A Potential Use In Immunodiagnosis. Elsevier Advanced Technology, v. 70, p. 275-281 AUG-2015.
Rights: embargo
Identifier DOI: 10.1016/j.bios.2015.03.034
Address: http://www.sciencedirect.com/science/article/pii/S0956566315001864
Date Issue: 2015
Appears in Collections:Unicamp - Artigos e Outros Documentos

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