Please use this identifier to cite or link to this item: http://repositorio.unicamp.br/jspui/handle/REPOSIP/243986
Type: Artigo
Title: Improvement in the expression of hepatic genes involved in fatty acid metabolism in obese rats supplemented with taurine
Author: Bonfleur, Maria Lúcia
Borck, Patrícia Cristine
Ribeiro, Rosane Aparecida
Caetano, Luiz Carlos
Soares, Gabriela Moreira
Carneiro, Everardo Magalhães
Balbo, Sandra Lucinei
Abstract: Aims: Fat deposition in the liver, which leads to nonalcoholic fatty liver disease is associated with obesity. Taurine (Tau) regulates lipid metabolism, representing a possible nutraceutical agent against obesity and its comorbidities. Here, we investigated whether Tau supplementation prevents hepatic lipid accumulation by regulation of the main hepatic genes involved in de nova lipogenesis and beta-oxidation. Main methods: Male rats received subcutaneous injections of monosodium glutamate (MSG; 4 mg/kg body weight/day) or saline (control group, CTL) during the first 5 days of life. From 21 to 120 days of age, half of each of the MSG and CTL groups received 2.5% Tau in drinking water (CTau and MTau). Key findings: MSG-treated rats were normoglycemic, hypertriglyceridemic and insulin resistant (IR). MSG rats also exhibited massive obesity and higher hepatic triglyceride (TG) content. This effect was associated with enhanced gene expression of fatty acid synthase (FASN), but reduced carbohydrate response element-binding protein (ChREBP), microsomal TG transfer protein (MTP) and carnitine palmitoyltransferase (CPT)-1a mRNAs in MSG livers. Tau supplementation decreased whole body fat accumulation and serum TG levels, without altering IR. Tau also normalized hepatic TG content by enhancing ChREBP, MTP, peroxisome proliferator-activated receptor (PPAR)-alpha, ACO (acyl-CoA oxidase) and CPT-1a gene expressions. Significance: Therefore, increased hepatic TG deposition in MSG-obese rats is associated with an enhanced FASN, and reduced MTP and CPT-1a genes. Tau supplementation prevented obesity and hepatic TG deposition by upregulating MTP mRNA, ameliorating hepatic lipid efflux, and consequently enhancing PPAR-alpha which increases lipid oxidation through ACO and CPT-1a gene expressions. (C) 2015 Elsevier Inc. All rights reserved.
Fat deposition in the liver, which leads to nonalcoholic fatty liver disease is associated with obesity. Taurine (Tau) regulates lipid metabolism, representing a possible nutraceutical agent against obesity and its comorbidities. Here, we investigated whether Tau supplementation prevents hepatic lipid accumulation by regulation of the main hepatic genes involved in de novo lipogenesis and beta-oxidation. Male rats received subcutaneous injections of monosodium glutamate (MSG; 4 mg/kg body weight/day) or saline (control group, CTL) during the first 5 days of life. From 21 to 120 days of age, half of each of the MSG and CTL groups received 2.5% Tau in drinking water (CTau and MTau). MSG-treated rats were normoglycemic, hypertriglyceridemic and insulin resistant (IR). MSG rats also exhibited massive obesity and higher hepatic triglyceride (TG) content. This effect was associated with enhanced gene expression of fatty acid synthase (FASN), but reduced carbohydrate response element-binding protein (ChREBP), microsomal TG transfer protein (MTP) and carnitine palmitoyltransferase (CPT)-1a mRNAs in MSG livers. Tau supplementation decreased whole body fat accumulation and serum TG levels, without altering IR. Tau also normalized hepatic TG content by enhancing ChREBP, MTP, peroxisome proliferator-activated receptor (PPAR)-alpha, ACO (acyl-CoA oxidase) and CPT-1a gene expressions. Therefore, increased hepatic TG deposition in MSG-obese rats is associated with an enhanced FASN, and reduced MTP and CPT-1a genes. Tau supplementation prevented obesity and hepatic TG deposition by upregulating MTP mRNA, ameliorating hepatic lipid efflux, and consequently enhancing PPAR-alpha which increases lipid oxidation through ACO and CPT-1a gene expressions
Subject: Acil-CoA oxidase
Abetalipoproteinemia
Glutamato monossódico
PPAR alfa
Carnitina O-palmitoiltransferase
Taurina
Country: Estados Unidos
Editor: Elsevier
Citation: Improvement In The Expression Of Hepatic Genes Involved In Fatty Acid Metabolism In Obese Rats Supplemented With Taurine. Pergamon-elsevier Science Ltd, v. 135, p. 15-21 AUG-2015.
Rights: fechado
Identifier DOI: 10.1016/j.lfs.2015.05.019
Address: http://www.sciencedirect.com/science/article/pii/S0024320515003100
Date Issue: 2015
Appears in Collections:IB - Artigos e Outros Documentos

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