Please use this identifier to cite or link to this item: http://repositorio.unicamp.br/jspui/handle/REPOSIP/243456
Type: Artigo de periódico
Title: Effects Of High Molecular Weight Hyaluronic Acid On Induced Arthritis Of The Temporomandibular Joint In Rats
Author: Lemos
George Azevedo; Rissi
Renato; Pimentel
Edson Rosa; Palomari
Evanisi Teresa
Abstract: High molecular weight hyaluronic acid (HMWHA) has been used to treat temporomandibular joint (TMJ) disorders, but controversial results have been described. This study aimed to characterize the morphological and biochemical actions of HMWHA on induced arthritis of the TMJ. Twenty-four male Wistar rats were used, and arthritis of the TMJ was induced through an intra-articular injection of Complete Freund's Adjuvant (CFA) (50 mu l). One week after arthritis induction, the animals were treated with HMWHA (once per week for three weeks). Histological analyses were performed using sections stained with hematoxylin-eosin, toluidine blue and Picrosirius. Were also performed histomorphometric analysis and birefringence of collagenous fibers (polarization microscopy). Biochemical analyses of TMJ tissues were carried out through measurements of sulfated glycosaminoglycans and zymography for evaluation of metalloproteinase-2 and -9 (MMP-2 and -9). Data were analyzed using paired t-test and unpaired t-test, with a 5% significance level. HMWHA reduced histologic changes and thickness of the articular disc, led to a greater arrangement of collagenous fibers, lower concentration of sulfated glycosaminoglycans and lower activity in all isoforms of MMP-2 and-gin TMJs with induced arthritis. These findings suggest that HMWHA may exert a protective effect on the TMJ. (C) 2015 Elsevier GmbH. All rights reserved.
Subject: Urokinase Plasminogen-activator
G-f 20
Intraarticular Injection
Early-stage
Polarization Microscopy
Osteoarthritis
Inflammation
Expression
Cartilage
Knee
Country: JENA
Editor: ELSEVIER GMBH, URBAN & FISCHER VERLAG
Rights: embargo
Identifier DOI: 10.1016/j.acthis.2015.05.003
Address: http://www.sciencedirect.com/science/article/pii/S0065128115000987
Date Issue: 2015
Appears in Collections:Unicamp - Artigos e Outros Documentos

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