Please use this identifier to cite or link to this item: http://repositorio.unicamp.br/jspui/handle/REPOSIP/243264
Type: Artigo de periódico
Title: Overexpression And Characterization Of The C-terminal Domain Of Human Siva1, A Proapoptotic Factor And Cytoskeleton Binding Protein
Author: Dantas
Larissa E. C.; Saad
Sara T. O.; Ramos
Carlos H. I.; Benichou
Serge
Abstract: Siva1 protein interacts with tumor protein p53 and with the member of the tumor necrosis factor receptor superfamily, stathmin, among others. These proteins are related to several pathways involved in cancer and are therefore strong candidate targets for drug design. This study aimed to characterize the biophysical properties of Siva 1 C-terminal domain to contribute to the discovery of new target directed drugs. The C-terminus Siva1 domain (residues 84-175) was fused to glutathione Stransferase (GST) and expressed in an E coli system and the recombinant GST-Siva C-terminus was purified by GST-Tagged Protein affinity and gel filtration chromatography. We tested the biological activity of the purified Siva C-terminus domain in a Jurkat extract cell line and found that the protein interacted with natural binders. Biophysical and biochemical assays have demonstrated monodispersion of the protein in solution with a predominant unfolded and elongated shape. However, at high concentrations, the protein showed a tendency to form soluble aggregates. These results are expected to lead to further progress in the understanding of Siva1 properties and target-directed drug design.
Subject: Analytical Ultracentrifugation
I-tasser
Apoptosis
Receptor
Cells
Prediction
Tryptophan
Multiple
Clustal
Size
Country: SHARJAH
Editor: BENTHAM SCIENCE PUBL LTD
Citation: Overexpression And Characterization Of The C-terminal Domain Of Human Siva1, A Proapoptotic Factor And Cytoskeleton Binding Protein. Bentham Science Publ Ltd, v. 23, p. 43-50 2016.
Rights: fechado
Identifier DOI: 10.2174/0929866522666151026122539
Address: http://benthamscience.com/journals/protein-and-peptide-letters/volume/23/issue/1/page/43/
Date Issue: 2016
Appears in Collections:Unicamp - Artigos e Outros Documentos

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