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Type: Artigo de periódico
Title: Virulence And Pathogenicity Of Candida Albicans Is Enhanced In Biofilms Containing Oral Bacteria
Author: Cavalcanti
Yuri Wanderley; Morse
Daniel James; da Silva
Wander Jose; Del-Bel-Cury
Altair Antoninha; Wei
Xiaoqing; Wilson
Melanie; Milward
Paul; Lewis
Michael; Bradshaw
David; Williams
David Wynne
Abstract: This study examined the influence of bacteria on the virulence and pathogenicity of candidal biofilms. Mature biofilms (Candida albicans-only, bacteria-only, C. albicans with bacteria) were generated on acrylic and either analysed directly, or used to infect a reconstituted human oral epithelium (RHOE). Analyses included Candida hyphae enumeration and assessment of Candida virulence gene expression. Lactate dehydrogenase (LDH) activity and Candida tissue invasion following biofilm infection of the RHOE were also measured. Candida hyphae were more prevalent (p < 0.05) in acrylic biofilms also containing bacteria, with genes encoding secreted aspartyl-proteinases (SAP4/SAP6) and hyphal-wall protein (HWP1) up-regulated (p < 0.05). Candida adhesin genes (ALS3/EPA1), SAP6 and HWP1 were up-regulated in mixed-species biofilm infections of RHOE. Multi-species infections exhibited higher hyphal proportions (p < 0.05), up-regulation of IL-18, higher LDH activity and tissue invasion. As the presence of bacteria in acrylic biofilms promoted Candida virulence, consideration should be given to the bacterial component when managing denture biofilm associated candidoses.
Subject: Pseudomonas-aeruginosa
Inflammatory Response
Citation: Virulence And Pathogenicity Of Candida Albicans Is Enhanced In Biofilms Containing Oral Bacteria. Taylor & Francis Ltd, v. 31, p. 27-38 2015.
Rights: embargo
Identifier DOI: 10.1080/08927014.2014.996143
Date Issue: 2015
Appears in Collections:Unicamp - Artigos e Outros Documentos

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